Wang D Y, Clement P, Smitz J, De Waele M, Derde M P
Department of Otorhinolaryngology, University Hospital, Free University Brussels, Belgium.
Allergol Immunopathol (Madr). 1994 Jul-Aug;22(4):179-83.
By using the microsuction technique, a quantitative determination of eosinophil cationic protein (ECP) in nasal secretions was performed in 18 atopic patients and 10 healthy volunteers. The percentage of the eosinophils was counted by simple microscopic examination. In the control group, results showed that the percentage of the eosinophils was almost 0 (range: 0-0.7%), and the median baseline ECP concentration was measured at 105 ng/g (range: 2-281 ng/g). No significant changes in either eosinophils or ECP were observed in this group. In the patient group, there existed a significantly higher baseline percentage of eosinophils (median: 2.7%, range: 0-45%) than in the control group but not for baseline ECP concentration (median: 160 ng/g, range: 6-1475 ng/g). One hour after nasal allergen challenge (NAC), a significant (P < 0.01) increase was seen in the percentage of eosinophils of the nasal secretions and this was remained for 10 hours (median: 5.5-13.3%). However, the difference that occurred was that ECP reached its highest concentration (median: 401 ng/g, range: 32-2298 ng/g) only 24 hours after NAC, while the percentage of the eosinophils decreased. Our findings again support a role of migration and activation of eosinophils in the pathogenesis of nasal allergic reaction, especially during the late phase.
通过使用微量抽吸技术，对18例特应性患者和10名健康志愿者的鼻分泌物中的嗜酸性粒细胞阳离子蛋白（ECP）进行了定量测定。通过简单的显微镜检查对嗜酸性粒细胞的百分比进行计数。在对照组中，结果显示嗜酸性粒细胞的百分比几乎为0（范围：0 - 0.7%），基线ECP浓度中位数为105 ng/g（范围：2 - 281 ng/g）。该组中嗜酸性粒细胞或ECP均未观察到显著变化。在患者组中，嗜酸性粒细胞的基线百分比（中位数：2.7%，范围：0 - 45%）显著高于对照组，但基线ECP浓度并非如此（中位数：160 ng/g，范围：6 - 1475 ng/g）。鼻内过敏原激发（NAC）1小时后，鼻分泌物中嗜酸性粒细胞的百分比显著增加（P < 0.01），并持续10小时（中位数：5.5 - 13.3%）。然而，不同的是，ECP仅在NAC后24小时达到其最高浓度（中位数：401 ng/g，范围：32 - 2298 ng/g），而嗜酸性粒细胞的百分比则下降。我们的研究结果再次支持嗜酸性粒细胞的迁移和激活在鼻过敏反应发病机制中的作用，尤其是在晚期。
