Thies F, Pillon C, Moliere P, Lagarde M, Lecerf J
Institut National de la Santé et de la Recherche Médicale U 352, Institut National des Sciences Appliquées-Lyon, Villeurbanne, France.
Am J Physiol. 1994 Nov;267(5 Pt 2):R1273-9. doi: 10.1152/ajpregu.1994.267.5.R1273.
The uptake and metabolism of [3H]docosahexaenoic acid (DHA) esterified at the sn-2 position of lysophosphatidylcholine (lysoPC DHA) and in the unesterified form, both bound to albumin, was studied in 20-day-old rats. LysoPC DHA was preferentially recovered in the brain (4-5% of the injected radioactivity) over the unesterified form of DHA (0.3-0.4%). Conversely, the lysoPC form was taken up less than or at the same extent as the unesterified form by the liver, heart, and kidney. In the brain, DHA was mainly recovered in phosphatidylethanolamine whether the esterified or the unesterified form was used, although DHA from lysoPC was esterified at the same extent in phosphatidylcholine and phosphatidylethanolamine after 2.5 min. The uptake of labeled palmitic, oleic, linoleic, and arachidonic acids, esterified or not in lysophosphatidylcholine, was also studied in brain, liver, heart, and kidney. Only the brain preferentially incorporated unsaturated (but not saturated) lysoPC, with the uptake increasing with the degree of unsaturation of the fatty acid moiety. These results strongly suggest that the young rat brain specifically utilizes albumin-lysoPC-containing polyunsaturated fatty acids.
在20日龄大鼠中研究了与白蛋白结合的、sn-2位酯化的[3H]二十二碳六烯酸（DHA）（溶血磷脂酰胆碱DHA，lysoPC DHA）和未酯化形式的DHA的摄取和代谢。与未酯化形式的DHA（0.3 - 0.4%）相比，lysoPC DHA在脑中的回收量更高（占注射放射性的4 - 5%）。相反，肝脏、心脏和肾脏对lysoPC形式的摄取量低于或与未酯化形式相同。在脑中，无论使用酯化形式还是未酯化形式的DHA，DHA主要在磷脂酰乙醇胺中回收，尽管2.5分钟后lysoPC中的DHA在磷脂酰胆碱和磷脂酰乙醇胺中的酯化程度相同。还研究了在溶血磷脂酰胆碱中酯化或未酯化的标记棕榈酸、油酸、亚油酸和花生四烯酸在脑、肝脏、心脏和肾脏中的摄取。只有脑优先摄取不饱和（而非饱和）的lysoPC，摄取量随脂肪酸部分不饱和程度的增加而增加。这些结果强烈表明，幼鼠脑特异性地利用含白蛋白-lysoPC 的多不饱和脂肪酸。
