Molina P E, Jabbour K, Williams P, Abumrad N N
Department of Surgery, Vanderbilt University Medical Center, Nashville, Tennessee 37232.
Am J Physiol. 1994 Nov;267(5 Pt 2):R1280-7. doi: 10.1152/ajpregu.1994.267.5.R1280.
This study examined the interaction of acute ethanol administration on glucose homeostasis during insulin-induced hypoglycemia (IIH). Glucose kinetics and net hepatic balances of alanine, glycerol, and lactate were estimated in three groups of dogs: group I underwent 3 h of IIH; group II received a continuous ethanol infusion in addition to IIH; group III received ethanol for 3 h. In group I, IIH resulted in a sustained twofold increase in glucose rate of appearance (Ra) and net hepatic uptake (NHU) of glycerol and lactate but no change in NHU of alanine. In group II, glucose Ra rose transiently, NHU of alanine and glycerol increased two- and fivefold, respectively, whereas NHU of lactate dropped 60%. In group III, no alterations in plasma glucose levels, glucose Ra, and NHU of alanine and glycerol were observed, but NHU of lactate switched to a net output. These results demonstrate that ethanol alone or with IIH exclusively inhibited NHU of lactate, and this was responsible, in a stoichiometric fashion, for the failure of the delayed increase in glucose Ra during IIH.
本研究检测了急性给予乙醇对胰岛素诱导的低血糖症（IIH）期间葡萄糖稳态的影响。在三组犬中评估了葡萄糖动力学以及丙氨酸、甘油和乳酸的肝脏净平衡：第一组经历3小时的IIH；第二组除IIH外还接受持续乙醇输注；第三组接受乙醇输注3小时。在第一组中，IIH导致葡萄糖出现率（Ra）以及甘油和乳酸的肝脏净摄取（NHU）持续增加两倍，但丙氨酸的NHU没有变化。在第二组中，葡萄糖Ra短暂升高，丙氨酸和甘油的NHU分别增加两倍和五倍，而乳酸的NHU下降60%。在第三组中，未观察到血浆葡萄糖水平、葡萄糖Ra以及丙氨酸和甘油的NHU有变化，但乳酸的NHU转变为净输出。这些结果表明，单独给予乙醇或与IIH一起给予乙醇均专门抑制了乳酸的NHU，并且这以化学计量方式导致了IIH期间葡萄糖Ra延迟增加的失败。
