Meacock S C, Brandon D R, Billingham M E
Lilly Research Centre Limited, Windlesham, Surrey, United Kingdom.
Ann Rheum Dis. 1994 Oct;53(10):653-8. doi: 10.1136/ard.53.10.653.
The role of lymphocytes and macrophages in developing adjuvant arthritis induced by an injection of CP20961 in inbred Lewis rats was studied over a 32 day period using a novel biotin-avidin immunoperoxidase histochemical technique.
Fresh frozen sections of hind paws and spleens, as well as lymph nodes draining the site of the injected adjuvant were immunostained using a panel of monoclonal antibodies specific for subsets of lymphocytes and macrophages and for MHC Class II antigen.
An increase in the numbers of activated T-lymphocytes was detected early in the draining lymph nodes before hind paw swelling had begun. The presence of these cells in significant numbers was only observed in the vicinity of the joint after joint swelling and damage had begun. Macrophages were among the first cells to invade the swollen paws and later were found with T-lymphocytes and cells bearing the MHC class II antigen at the face of eroding and re-organising bone.
The activity of T-lymphocytes in initiating arthritis appeared to occur early in lymph nodes. Joint destruction was more closely associated with the arrival of macrophages but later arrival of T-lymphocytes may have contributed to the maintenance of chronic inflammation.
采用一种新型生物素-抗生物素蛋白免疫过氧化物酶组织化学技术，在32天的时间里研究淋巴细胞和巨噬细胞在近交系Lewis大鼠注射CP20961诱导的佐剂性关节炎发展过程中的作用。
使用一组针对淋巴细胞和巨噬细胞亚群以及MHC II类抗原的单克隆抗体，对后爪、脾脏的新鲜冰冻切片以及注射佐剂部位引流的淋巴结进行免疫染色。
在后爪肿胀开始之前，在引流淋巴结中早期就检测到活化T淋巴细胞数量增加。只有在关节肿胀和损伤开始后，才在关节附近观察到大量这些细胞的存在。巨噬细胞是最早侵入肿胀后爪的细胞之一，后来在侵蚀和重新组织的骨表面发现它们与T淋巴细胞和携带MHC II类抗原的细胞在一起。
T淋巴细胞引发关节炎的活动似乎在淋巴结中早期就发生了。关节破坏与巨噬细胞的到来关系更为密切，但T淋巴细胞较晚到来可能有助于维持慢性炎症。
