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[The use of a protoplast-generating method in selecting the lytic enzyme producer Streptomyces recifensis subsp. lyticus].

作者信息

Kulikova T Ia, Sten'ko A S, Bezkorovaĭnaia N K, Babenko Iu S

出版信息

Antibiot Khimioter. 1994 Feb-Mar;39(2-3):9-12.

PMID:7979802
Abstract

Optimal conditions for the formation of stable protoplasts of S. recifensis subsp. lyticus 2435 were defined and the use of the protoplasting procedure to increase the activity of the synthesis of extracellular enzymes was shown in principle possible. The level of the synthesis of glycosidases and lytic endopeptidases by the isolated variant II-29 was higher than that the initial strain by 40-45 per cent which was due to the enrichment of the population with more active variants.

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