A biosynthesis activity of Streptomyces recifensis var. lyticus.

The structure of enzyme complex synthesized of soil streptomycete was investigated. Fractionation and purification of enzymes have allowed to identify a line of hydrolases: proteinases, amylases, DNA-ses, cellulases and also glycosidases and endopeptidases with lytic action. Their properties were studied. The isolated lytic endopeptidases had a wide substrate specificity and hydrolyzed casein and synthetic peptides at pH 7-9 and also a cell wall of bacteria at pH 5-6. The temperature optimum of 5 researched endopeptidases are in the range 45-60 degrees C. They were activated by ions Co2+, Fe2+, Cu2+ to a different degree. The universal activators of lytic endopeptidases were ions Cd2+ and Pb2+. They have raised lytic activity of enzymes 3-7 times. The obtained data can witness high adaptogenic ability of streptomycetes in relation to heavy metals. The possibility of enzyme preparation from Streptomyces recifensis use for growth stimulation of agricultural plants was shown also against the wheat, oats, maize, haricot beans, peas, barley.