在痘苗病毒/T7/脑心肌炎病毒系统中表达的甲型肝炎病毒核衣壳的物理化学和免疫学特性
Physiochemical and immunological characterization of hepatitis A virus nucleocapsids expressed in a vaccinia virus/T7/EMCV system.
作者信息
Zhu N L, Li G D, Wang Y
机构信息
Shanghai Institute of Biochemistry, Academia Sinica, People's Republic of China.
出版信息
Arch Virol. 1994;135(3-4):443-9. doi: 10.1007/BF01310029.
PMID:7979980
Abstract
Expression of the entire open reading frame of hepatitis A virus was achieved in a vaccinia virus/T7/EMCV hybrid system. The expressed antigens were characterized as particles with buoyant densities and sedimentation coefficients typical of empty capsids and pentamers of HAV. This was further confirmed by electron microscopy. All capsid proteins were determined as components of the particles which raised neutralizing antibodies in an immunized rabbit.
摘要
甲型肝炎病毒的整个开放阅读框在痘苗病毒/T7/脑心肌炎病毒杂交系统中得以表达。所表达的抗原被鉴定为具有甲型肝炎病毒空衣壳和五聚体典型的浮力密度和沉降系数的颗粒。电子显微镜进一步证实了这一点。所有衣壳蛋白都被确定为能在免疫兔中引发中和抗体的颗粒成分。
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