重组杆状病毒诱导的传染性胃肠炎病毒特异性抗体对仔猪的被动保护作用。
Passive protection of piglets by recombinant baculovirus induced transmissible gastroenteritis virus specific antibodies.
作者信息
Tuboly T, Nagy E, Derbyshire J B
机构信息
Department of Veterinary Microbiology and Immunology, Ontario Veterinary College, University of Guelph, Ontario.
出版信息
Can J Vet Res. 1995 Jan;59(1):70-2.
Sera of pigs immunized with parts of the transmissible gastroenteritis virus (TGEV) spike (S) protein expressed by recombinant baculoviruses were tested, together with a TGEV hyperimmune antiserum, for their abilities to protect three-day-old piglets against TGEV infection. The piglets were infected with virulent TGEV and the sera were given orally 3 h before infection, together with the virus, and every 6 h postinfection during the 30 h of the experiment. Virus shedding was monitored by TGEV isolation from rectal swab samples. The sera containing antibodies induced by the complete S protein or the amino terminal half of the S protein showed protective properties, indicated by delayed onset of clinical signs and virus shedding, similar to the TGEV hyperimmune serum. Those immune sera containing antibodies induced by shorter recombinant proteins were not protective.
用重组杆状病毒表达的传染性胃肠炎病毒（TGEV）刺突（S）蛋白的部分片段免疫猪的血清，与TGEV超免疫抗血清一起，检测它们保护三日龄仔猪免受TGEV感染的能力。仔猪感染强毒TGEV，在感染前3小时与病毒一起口服血清，在实验的30小时内感染后每6小时口服一次。通过从直肠拭子样本中分离TGEV来监测病毒排出。含有由完整S蛋白或S蛋白氨基末端一半诱导产生的抗体的血清表现出保护特性，表现为临床症状和病毒排出延迟，类似于TGEV超免疫血清。那些含有由较短重组蛋白诱导产生的抗体的免疫血清没有保护作用。
Zotero 插件