Mita E, Hayashi N, Iio S, Takehara T, Hijioka T, Kasahara A, Fusamoto H, Kamada T
First Department of Medicine, Osaka University School of Medicine, Japan.
Biochem Biophys Res Commun. 1994 Oct 28;204(2):468-74. doi: 10.1006/bbrc.1994.2483.
To investigate the role that Fas ligand plays in the apoptosis of hepatocytes induced by hepatitis C virus infection, we isolated a cDNA clone for human Fas ligand and examined the expression of Fas ligand in liver-infiltrating mononuclear cells obtained from patients with chronic hepatitis C. The amino acid sequence of human Fas ligand showed 76% and 77% identity with those of rat and mouse Fas ligand, respectively. When the expression of Fas ligand transcripts was tested by reverse transcription-polymerase chain reaction, the amplified signal was detected in liver-infiltrating mononuclear cells and peripheral blood mononuclear cells, whereas only a weak signal or none at all was detected in liver tissues. These findings suggest that the Fas ligand-Fas antigen system may play an important role in liver cell injury by hepatitis C virus infection.
为了研究Fas配体在丙型肝炎病毒感染诱导的肝细胞凋亡中所起的作用，我们分离出了人Fas配体的cDNA克隆，并检测了从慢性丙型肝炎患者获得的肝浸润单核细胞中Fas配体的表达。人Fas配体的氨基酸序列与大鼠和小鼠Fas配体的氨基酸序列分别有76%和77%的同源性。当通过逆转录-聚合酶链反应检测Fas配体转录本的表达时，在肝浸润单核细胞和外周血单核细胞中检测到了扩增信号，而在肝组织中仅检测到微弱信号或根本没有信号。这些发现表明，Fas配体-Fas抗原系统可能在丙型肝炎病毒感染导致的肝细胞损伤中起重要作用。
