O'Bryan M K, Baker H W, Saunders J R, Kirszbaum L, Walker I D, Hudson P, Liu D Y, Glew M D, d'Apice A J, Murphy B F
St. Vincent's Hospital, Melbourne, Australia.
J Clin Invest. 1990 May;85(5):1477-86. doi: 10.1172/JCI114594.
Molecular cloning of the human complement inhibitor SP-40,40, has revealed strong homology to a major rat and ram Sertoli cell product, sulfated glycoprotein-2, known also as clusterin. This study reports the purification and characterization of human seminal clusterin. Two-dimensional gel electrophoresis revealed charge differences between clusterin purified from semen and the serum-derived material. Both preparations demonstrate comparable hemagglutination (clustering) activity and inhibition of C5b-6 initiated hemolysis. The average clusterin concentration in normal seminal plasma is considerably higher than that found in serum. Mean seminal plasma clusterin concentrations were significantly lower in azoospermia caused by obstruction or seminiferous tubule failure than with oligospermia or normospermia. Only men with vasal agenesis had undetectable seminal clusterin, suggesting that some of the seminal clusterin is produced by the seminal vesicles. Immunofluorescence of human spermatozoa revealed that clusterin was detected on 10% of spermatozoa, predominantly those that were immature or had abnormal morphology. A pilot study of 25 patients suggests that seminal clusterin concentration, together with sperm motility and morphology, is correlated with the fertilization rate in vitro. The function of seminal clusterin is unknown. Its extensive distribution in the male genital tract and its high concentration in seminal plasma suggests an important role in male fertility.
人类补体抑制剂SP-40,40的分子克隆显示，它与大鼠和公羊主要的支持细胞产物硫酸化糖蛋白-2（也称为聚集素）具有高度同源性。本研究报告了人类精液聚集素的纯化和特性。二维凝胶电泳显示，从精液中纯化的聚集素与血清来源的物质在电荷上存在差异。两种制剂均表现出相当的血凝（聚集）活性以及对C5b-6引发的溶血的抑制作用。正常精浆中聚集素的平均浓度明显高于血清中的浓度。由梗阻或生精小管功能衰竭导致的无精子症患者的精浆聚集素平均浓度显著低于少精子症或正常精子症患者。只有输精管发育不全的男性精液中检测不到聚集素，这表明部分精液聚集素是由精囊产生的。对人类精子的免疫荧光检测显示，10%的精子上可检测到聚集素，主要是那些不成熟或形态异常的精子。对25名患者的初步研究表明，精液聚集素浓度与精子活力和形态一起，与体外受精率相关。精液聚集素的功能尚不清楚。它在男性生殖道中的广泛分布以及在精浆中的高浓度表明其在男性生育中起重要作用。
