Sienkiewicz Z J, Robbins L, Haylock R G, Saunders R D
National Radiological Protection Board, Didcot, Oxon, United Kingdom.
Bioelectromagnetics. 1994;15(4):363-75. doi: 10.1002/bem.2250150410.
To investigate the potential of magnetic fields to act as a behavioral teratogen, pregnant CD1 mice were exposed or sham-exposed for all of gestation to a 50 Hz/20 mT magnetic field. Maturation of offspring was assessed using a range of standard developmental indices (eye opening, pinna detachment, hair coat, tooth eruption, sexual maturity, and weight) and simple reflexive behaviors (air righting, surface righting, forepaw grasp, cliff avoidance, and negative geotaxis). Activity and coordination levels were explored in juvenile and adult mice using an open field arena, a head-dip board, an accelerating Rotarod, and a residential activity wheel. All assessments were carried out without knowledge of exposure condition. Results from 168 sham-exposed mice from 21 litters and from 184 exposed mice from 23 litters were compared using survival analysis techniques and multivariate regression methods. Three possible field-dependent effects were found: Exposed animals performed the air righting reflex earlier (P < 0.01); exposed males (but not females) were significantly lighter in weight (P = 0.008) at 30 days of age; and exposed animals remained on a Rota-rod for less time as juveniles (P = 0.03). Some of these results have not been reported in other studies and may reflect spurious statistical significance, although some effect of magnetic field exposure cannot be ruled out. Overall, these results suggest that prenatal exposure to a 50 Hz magnetic field does not engender any gross impairments in the postnatal development or behavior of mice. This does not preclude such exposure affecting more subtle aspects of behavior.
为了研究磁场作为行为致畸原的可能性，将怀孕的CD1小鼠在整个妊娠期暴露于50Hz/20mT的磁场中或进行假暴露。使用一系列标准发育指标（睁眼、耳廓分离、被毛、牙齿萌出、性成熟和体重）以及简单的反射行为（空中翻正、表面翻正、前爪抓握、悬崖回避和负趋地性）来评估后代的成熟情况。使用旷场竞技场、头部浸入板、加速转棒和居家活动轮来探究幼年和成年小鼠的活动和协调水平。所有评估均在不知道暴露情况的前提下进行。使用生存分析技术和多元回归方法比较了来自21窝的168只假暴露小鼠和来自23窝的184只暴露小鼠的结果。发现了三种可能的磁场依赖性效应：暴露组动物的空中翻正反射出现得更早（P<0.01）；30日龄时，暴露组雄性（而非雌性）体重显著更轻（P = 0.008）；暴露组幼年动物在转棒上停留的时间更短（P = 0.03）。这些结果中的一些在其他研究中尚未报道，可能反映了虚假的统计学显著性，尽管不能排除磁场暴露的某些影响。总体而言，这些结果表明，产前暴露于50Hz磁场不会对小鼠的产后发育或行为造成任何明显损害。但这并不排除这种暴露会影响行为的更细微方面。
