Amoura Z, Chabre H, Koutouzov S, Lotton C, Cabrespines A, Bach J F, Jacob L
Unité INSERM 25, Hôpital Necker, Paris, France.
Arthritis Rheum. 1994 Nov;37(11):1684-8. doi: 10.1002/art.1780371118.
To compare the humoral response to nucleosomes with the response to their individual components (double-stranded DNA [dsDNA] and histones) and to assess the involvement of antinucleosome antibodies in immune deposits in the kidney of MRL mice.
We used enzyme-linked immunosorbent assays of sera and kidney eluates for antibody activity against purified nucleosomes, dsDNA, and histones.
Antinucleosome antibodies emerged before anti-dsDNA and antihistone antibodies. A fraction of antinucleosome antibodies reacted exclusively with nucleosomes and not with their components, dsDNA and histones. These nucleosome-restricted antibodies were detected in the proteinuric MRL mouse kidney eluate.
Our findings support the notion that nucleosomes play a major role in the emergence of antinuclear autoantibodies and that antinucleosome antibodies might be involved in the nephritogenic process in murine lupus.
比较机体对核小体的体液免疫反应与其对各组分（双链DNA[dsDNA]和组蛋白）的反应，并评估抗核小体抗体在MRL小鼠肾脏免疫沉积物中的作用。
我们采用酶联免疫吸附测定法检测血清和肾脏洗脱液中针对纯化核小体、dsDNA和组蛋白的抗体活性。
抗核小体抗体比抗dsDNA和抗组蛋白抗体出现得早。一部分抗核小体抗体仅与核小体反应，而不与dsDNA和组蛋白这些组分反应。在出现蛋白尿的MRL小鼠肾脏洗脱液中检测到了这些核小体特异性抗体。
我们的研究结果支持以下观点，即核小体在抗核自身抗体的产生中起主要作用，并且抗核小体抗体可能参与了小鼠狼疮的致肾炎过程。
