Chabre H, Amoura Z, Piette J C, Godeau P, Bach J F, Koutouzov S
Hôpital Necker, Paris, France.
Arthritis Rheum. 1995 Oct;38(10):1485-91. doi: 10.1002/art.1780381015.
To assess whether nucleosome-restricted antibodies, i.e., antibodies that react with the whole nucleosome particle but not with its individual components (double-stranded DNA [dsDNA] and histones), are present in the sera of patients with systemic lupus erythematosus (SLE).
Antibodies were detected by enzyme-linked immunosorbent assay using purified nucleosomes, dsDNA, or histones. These tests were applied to the sera of 40 patients with SLE. Protein G-purified IgGs of representative sera were sequentially adsorbed on dsDNA- and histone-conjugated solid-phase supports and further assayed for their nucleosome, dsDNA, and histone reactivities.
Of the 40 sera tested, 16 displayed anti-dsDNA and/or antihistone antibody activity, which was always associated with significant antinucleosome reactivity. In addition, 3 sera showed antinucleosome activity that was not associated with concomitant anti-dsDNA or antihistone activity. The presence of true nucleosome-restricted antibodies was demonstrated, after solid-phase adsorption, in representative SLE sera that showed anti-dsDNA or antihistone antibody activity, and also in sera that did not show these activities.
Our results provide evidence for the presence of nucleosome-restricted antibodies in patients with lupus. These nucleosome-restricted antibodies, along with anti-dsDNA and antihistone antibodies, appear to belong to a broad set of antinuclear antibodies, the antinucleosome family.
评估系统性红斑狼疮（SLE）患者血清中是否存在核小体限制性抗体，即与整个核小体颗粒发生反应但不与其单个成分（双链DNA [dsDNA] 和组蛋白）发生反应的抗体。
使用纯化的核小体、dsDNA或组蛋白通过酶联免疫吸附测定法检测抗体。这些检测应用于40例SLE患者的血清。将代表性血清经蛋白G纯化的IgG依次吸附在dsDNA和组蛋白偶联的固相支持物上，并进一步检测其对核小体、dsDNA和组蛋白的反应性。
在检测的40份血清中，16份显示出抗dsDNA和/或抗组蛋白抗体活性，且总是与显著的抗核小体反应性相关。此外，3份血清显示出抗核小体活性，但与同时存在的抗dsDNA或抗组蛋白活性无关。在显示抗dsDNA或抗组蛋白抗体活性的代表性SLE血清中，以及在未显示这些活性的血清中，经固相吸附后证实存在真正的核小体限制性抗体。
我们的结果为狼疮患者中存在核小体限制性抗体提供了证据。这些核小体限制性抗体与抗dsDNA和抗组蛋白抗体一起，似乎属于一组广泛的抗核抗体，即抗核小体家族。
