Blank A L, Davis G L, VanDeWater T R, Ruben R J
Department of Otolaryngology, Albert Einstein College of Medicine, Bronx, NY.
Arch Otolaryngol Head Neck Surg. 1994 Dec;120(12):1342-6. doi: 10.1001/archotol.1994.01880360040008.
To create an experimental model of Streptococcus pneumoniae type 3 meningogenic labyrinthitis (a leading cause of deafness) similar to that in human disease.
Cohort analytic study of guinea pigs that were inoculated intrathecally with varying dilutions of S pneumoniae type 3; the progress of the disease was compared with that in saline solution-inoculated control animals.
Healthy adult Hartley guinea pigs without clinical evidence of middle ear disease that were conveniently sampled.
Intrathecal inoculation of 10(4) to 10(6) colony-forming units of S pneumoniae type 3 into 13 guinea pigs; signs and symptoms of meningitis/labyrinthitis were observed for 15 days and compared with those in two saline solution-inoculated control animals.
Morbidity--labyrinthitis, meningitis; end point--death.
The 10(4) to 10(6) colony-forming units of S pneumoniae type 3 caused inflammation that extended from the meninges to the inner ear via the cochlear aqueduct within 3 days after inoculation; a dose of 10(7) killed animals within 12 hours after inoculation. Three of five animals that were inoculated with a 10(6) dose died 3 days after inoculation; two of three animals that were inoculated with a 10(5) dose lived to 15 days after inoculation. One of two animals that were inoculated with a 10(4) dose did not become infected. Inflammation extended to the middle ear by round-window destruction. Reactive bone formation simulated labyrinthine osteosclerosis. Observers assessed histologic slides "blindly."
Guinea pigs can survive 15 days after intrathecal inoculation of a 10(5) dose, with morphologic features similar to those in human disease. This is an effective model for this study of meningogenic labyrinthitis.
建立一种类似于人类疾病的3型肺炎链球菌致脑膜炎性迷路炎（耳聋的主要原因）实验模型。
对鞘内接种不同稀释度3型肺炎链球菌的豚鼠进行队列分析研究；将疾病进展与接种盐溶液的对照动物进行比较。
方便抽样的无中耳疾病临床证据的健康成年哈特利豚鼠。
对13只豚鼠鞘内接种10⁴至10⁶个3型肺炎链球菌菌落形成单位；观察15天的脑膜炎/迷路炎体征和症状，并与两只接种盐溶液的对照动物进行比较。
发病率——迷路炎、脑膜炎；终点——死亡。
接种后3天内，10⁴至10⁶个3型肺炎链球菌菌落形成单位引起的炎症从脑膜经蜗水管扩展至内耳；10⁷剂量在接种后12小时内导致动物死亡。接种10⁶剂量的五只动物中有三只在接种后3天死亡；接种10⁵剂量的三只动物中有两只存活至接种后15天。接种10⁴剂量的两只动物中有一只未被感染。炎症通过圆窗破坏扩展至中耳。反应性骨形成模拟迷路骨硬化。观察者“盲法”评估组织学切片。
豚鼠鞘内接种10⁵剂量后可存活15天，其形态学特征与人类疾病相似。这是该致脑膜炎性迷路炎研究的有效模型。
