Co-amplification of two cDNAs in RT-PCR can alter the yield of both products.
作者信息
Braga V M, Gendler S J
机构信息
Imperial Cancer Research Fund, London, UK.
出版信息
Biotechniques. 1994 Aug;17(2):228-30.
PMID:7980911
Abstract
摘要
相似文献
1
Co-amplification of two cDNAs in RT-PCR can alter the yield of both products.
Biotechniques. 1994 Aug;17(2):228-30.
2
Two-step PCR amplification of multiple specific products from cDNA using one specific primer and oligo dT.使用一个特异性引物和寡聚dT从cDNA中进行多特异性产物的两步PCR扩增。
Biotechniques. 1994 Feb;16(2):251-2.
3
Amplification of cDNA ends using PCR suppression effect and step-out PCR.利用PCR抑制效应和步移PCR扩增cDNA末端
Methods Mol Biol. 2003;221:41-9. doi: 10.1385/1-59259-359-3:41.
4
Multiple mutant cDNAs from one reaction mixture using asymmetric primers in PCR.在聚合酶链式反应（PCR）中使用不对称引物从一个反应混合物中获得多个突变互补脱氧核糖核酸（cDNA）。
Biotechniques. 1995 Oct;19(4):556-9.
5
Chromosomal assignment of 79 cDNAs from a range of human tissues.来自一系列人体组织的79个cDNA的染色体定位。
Genomics. 1996 Jan 1;31(1):130-4. doi: 10.1006/geno.1996.0021.
6
One-tube RT-PCR with sequence-specific primers.使用序列特异性引物的单管逆转录聚合酶链反应。
Methods Mol Biol. 1998;86:143-51. doi: 10.1385/0-89603-494-1:143.
7
An end-trimming method to amplify adjacent cDNA fragments by PCR.
Biotechniques. 1994 Jan;16(1):94-8.
8
A simple and rapid protocol for the sequence determination of functional kappa light chain cDNAs from aberrant-chain-positive murine hybridomas.一种用于从异常链阳性小鼠杂交瘤中测定功能性κ轻链cDNA序列的简单快速方法。
J Immunol Methods. 2004 Nov;294(1-2):199-207. doi: 10.1016/j.jim.2004.09.001. Epub 2004 Oct 4.
9
Construction of long-transcript enriched cDNA libraries from submicrogram amounts of total RNAs by a universal PCR amplification method.通过通用PCR扩增方法从亚微克量的总RNA构建富含长转录本的cDNA文库。
Genome Res. 2001 Sep;11(9):1553-8. doi: 10.1101/gr.185501.
10
Reverse transcription and PCR amplification of rare mRNAs immobilized on oligo(dT) cellulose.固定在寡聚（dT）纤维素上的稀有mRNA的逆转录和PCR扩增。
Anal Biochem. 1993 Aug 15;213(1):170-1. doi: 10.1006/abio.1993.1402.
引用本文的文献
1
PCR in situ: aspects which reduce amplification and generate false-positive results.原位聚合酶链反应：降低扩增并产生假阳性结果的因素。
Histochem J. 1995 Sep;27(9):660-9.
Zotero 插件