HRRI：一种用于原位杂交实验中快速、直接定量的高分辨率放射成像仪。

HRRI: a high resolution radioimager for fast, direct quantification in in situ hybridization experiments.

作者信息

Lanièce P, Charon Y, Dumas S, Mastrippolito R, Pinot L, Tricoire H, Valentin L

机构信息

Institut de Physique Nucléaire, CNRS, Orsay, France.

出版信息

Biotechniques. 1994 Aug;17(2):338-45.

PMID:7980938

Abstract

We present a high-speed, high-resolution beta imager. It has been developed to be used in in situ hybridization experiments, either instead of or in complement with autoradiographic film and emulsions that are currently used for these experiments. It allows the user to locate and perform quantitative analyses of (3H-, 14C-, 35S-, 32P-, 125I-) labeled molecules with a 15-microns spatial resolution on a 1.2 cm2 area. We have combined recent techniques (specific scintillator thin sheets and intensified charge-coupled device [CCD]) so that this imager offers a wide dynamic range and real-time acquisition. Several biological applications will be discussed.

摘要

我们展示了一种高速、高分辨率的β成像仪。它被开发用于原位杂交实验，可替代或补充目前用于这些实验的放射自显影片和乳剂。它允许用户在1.2平方厘米的区域内以15微米的空间分辨率对（3H、14C、35S、32P、125I）标记的分子进行定位和定量分析。我们结合了最新技术（特定的闪烁体薄片和增强型电荷耦合器件[CCD]），使得该成像仪具有宽动态范围和实时采集功能。还将讨论几种生物学应用。