Singletary S E, Baker F L, Spitzer G, Tucker S L, Tomasovic B, Brock W A, Ajani J A, Kelly A M
Cancer Res. 1987 Jan 15;47(2):403-6.
The effect of epidermal growth factor (EGF) on the in vitro growth of 186 malignant human tumor specimens (45 melanomas, 32 sarcomas, and 56 lung, 16 gynecological, 14 breast, 12 genitourinary, and 11 gastrointestinal carcinomas) was evaluated in the cellular adhesive matrix human tumor culture system supplemented with transferrin, insulin, hydrocortisone, and estradiol. EGF increased tumor growth by at least 50% in 81% of the 186 tumors and by over 100% in 54%. The enhanced growth induced by EGF was related to an accelerated cellular division independent of tumor type and not to an increase in the actual number of clonogenic units. The drug concentrations of cell cycle-independent Adriamycin and cisplatin needed to achieve a 90% tumor cell kill were not altered by the responsiveness of the tumor to EGF.
在补充了转铁蛋白、胰岛素、氢化可的松和雌二醇的细胞粘附基质人肿瘤培养系统中，评估了表皮生长因子（EGF）对186例人类恶性肿瘤标本（45例黑色素瘤、32例肉瘤、56例肺癌、16例妇科肿瘤、14例乳腺癌、12例泌尿生殖系统肿瘤和11例胃肠道癌）体外生长的影响。在186例肿瘤中，81%的肿瘤中EGF使肿瘤生长至少增加50%，54%的肿瘤中EGF使肿瘤生长增加超过100%。EGF诱导的生长增强与细胞分裂加速有关，与肿瘤类型无关，而与克隆形成单位的实际数量增加无关。肿瘤对EGF的反应性并未改变细胞周期非依赖性药物阿霉素和顺铂达到90%肿瘤细胞杀伤所需的药物浓度。
