Vannucci R C, Brucklacher R M
Department of Pediatrics (Pediatric Neurology), Pennsylvania State University College of Medicine, Milton S. Hershey Medical Center, Hershey 17033.
Brain Res. 1994 Aug 8;653(1-2):141-7. doi: 10.1016/0006-8993(94)90382-4.
The brain mitochondrial NAD+/NADH ratio, as a reflection of the oxidation-reduction (redox) state of cellular compartment, was determined under conditions of hypoxia, anoxia, hypoxia-ischemia, complete ischemia and hypoglycemia in immature rats. NAD+/NADH ratios were calculated from changes in the concentrations of specific oxidative substrates and calculated intracellular pH during cerebral metabolic stress. The results suggest that the use of the acetoacetate/beta-hydroxybutyrate substrate couple provides a more accurate prediction of the mitochondrial redox state under adverse conditions than use of the alpha-ketoglutarate/glutamate couple. It is possible that the mitochondrial oxidation seen with the latter substrate couple during cerebral metabolic stress might reflect a population of cells (neurons or glia) which are substrate-deprived relative to the rest of the brain in the setting of metabolic stress produced by oxygen deficiency.
在未成熟大鼠中，于缺氧、无氧、缺氧缺血、完全缺血和低血糖条件下，测定了反映细胞区室氧化还原状态的脑线粒体NAD⁺/NADH比值。NAD⁺/NADH比值根据特定氧化底物浓度的变化以及脑代谢应激期间计算出的细胞内pH值来计算。结果表明，与使用α-酮戊二酸/谷氨酸对相比，使用乙酰乙酸/β-羟基丁酸底物对能更准确地预测不利条件下的线粒体氧化还原状态。在后一种底物对在脑代谢应激期间出现的线粒体氧化，可能反映了在缺氧产生的代谢应激情况下，相对于大脑其他部分而言底物缺乏的一群细胞（神经元或神经胶质细胞）。
