Milićević N M, Appasamy P M, Colić M, Milićević Z
Institute of Histology, Medical Faculty, University of Beograd, Yugoslavia.
Immunobiology. 1994 Jun;190(4-5):376-84. doi: 10.1016/s0171-2985(11)80609-1.
As revealed with ED1 and ED2 monoclonal antibodies, macrophages are scattered throughout the thymic tissue. However, in contrast to the cortex and medulla, in the cortico-medullary zone macrophages are large and show strong reactivity with rabbit polyclonal antisera to cyclooxygenase. Only few smaller cortical macrophages also show weaker presence of prostaglandin synthase. After cyclosporin treatment cortical macrophages become strikingly similar to the macrophages of the cortico-medullary zone of the normal thymus. Cortical macrophages become enlarged and develop the strong expression of prostaglandin synthase. Our results show that a specific type of macrophages (with distinct histochemical characteristics, enzyme profile and ultrastructural organization, which is strategically positioned within the thymic tissue--as we demonstrated earlier) possesses the enzyme capacity required for prostaglandin synthesis. After cyclosporin treatment, which interferes with the maturation of thymocytes, cortical macrophages thoroughly change and develop the strong prostaglandin synthase expression, similar to that of normal cortico-medullary zone macrophages.
用ED1和ED2单克隆抗体显示，巨噬细胞散在于整个胸腺组织中。然而，与皮质和髓质不同，在皮质-髓质区巨噬细胞较大，并且与兔抗环氧化酶多克隆抗血清显示出强烈反应性。只有少数较小的皮质巨噬细胞也显示出较弱的前列腺素合酶存在。环孢素治疗后，皮质巨噬细胞变得与正常胸腺皮质-髓质区的巨噬细胞极为相似。皮质巨噬细胞变大并出现前列腺素合酶的强烈表达。我们的结果表明，一种特定类型的巨噬细胞（具有独特的组织化学特征、酶谱和超微结构组织，正如我们之前所证明的，其在胸腺组织中具有战略定位）具备前列腺素合成所需的酶能力。环孢素治疗干扰胸腺细胞成熟后，皮质巨噬细胞彻底改变并出现强烈的前列腺素合酶表达，类似于正常皮质-髓质区巨噬细胞。
