Milićević N M, Milićević Z, Colić M
Institute of Histology, Medical Faculty, University of Beograd, Yugoslavia.
Virchows Arch B Cell Pathol Incl Mol Pathol. 1989;57(4):237-44. doi: 10.1007/BF02899087.
Young adult Wistar rats received 40 mg/kg of cyclosporin perorally for 21 days. Cyclosporin induced almost total disappearance of thymic medulla, whereas the cortex remained preserved. Although the density of cortical macrophages did not change significantly, their characteristics altered markedly and they became enlarged and rounded. In addition to an increase in acid phosphatase and nonspecific esterase activities, cortical macrophages developed very strong succinic dehydrogenase and chloroacetate esterase activities and a fine, granular, aldehyde fuchsin-positive cytoplasmic content. However, these cytoplasmic granules were PAS-negative and were not sudanophilic. Cortical macrophages retained their normal antigenic properties (which were studied by the use of ED1, ED2 and R-MC 41 monoclonal antibodies). Phagocytic cells in the remaining medullary islands retained their usual characteristics. The changes in cortical macrophages after cyclosporin treatment are discussed, especially in relation to the characteristics of macrophages of the cortico-medullary zone in the normal rat thymus.
将年轻成年Wistar大鼠口服给予40mg/kg环孢素，持续21天。环孢素导致胸腺髓质几乎完全消失，而皮质则保持完整。尽管皮质巨噬细胞的密度没有显著变化，但其特征发生了明显改变，变得增大且呈圆形。除了酸性磷酸酶和非特异性酯酶活性增加外，皮质巨噬细胞还产生了非常强的琥珀酸脱氢酶和氯乙酸酯酶活性，以及精细的、颗粒状的、醛复红阳性的细胞质内容物。然而，这些细胞质颗粒对过碘酸希夫反应（PAS）呈阴性，且不嗜苏丹染料。皮质巨噬细胞保留了其正常的抗原特性（通过使用ED1、ED2和R-MC 41单克隆抗体进行研究）。剩余髓质岛中的吞噬细胞保持其通常的特征。讨论了环孢素处理后皮质巨噬细胞的变化，特别是与正常大鼠胸腺皮质-髓质区巨噬细胞的特征相关的变化。
