Forsgren S, Franzén L, Liang Y, Gustafsson H, Henriksson R
Department of Anatomy, University of Umeå, Sweden.
Histochem J. 1994 Aug;26(8):630-40. doi: 10.1007/BF00158287.
It is well-known that a large number of factors can influence the expression of neuropeptides in the nervous system. In the present study, the effects of unilateral and bilateral irradiation to the rat head and neck on the expression of neuropeptides in the innervation of the submandibular gland and in the ganglionic cells of the submandibular ganglion was examined ten days and six months after treatment. Antisera directed against enkephalin and bombesin and immunohistochemical methods were used. The effects of bilateral irradiation on the staining pattern of various neuropeptides in the cervical spinal cord were also studied. In the submandibular gland and in the submandibular ganglionic cells, there was a markedly increased neuropeptide expression ten days after bilateral treatment, as seen after staining with both antisera used, while no changes occurred after unilateral treatment. Six months after treatment, the pattern of neuropeptide expression in the submandibular gland/ganglion corresponded to that seen in controls. Irradiation did not lead to any changes in the staining pattern of neuropeptides in the spinal cord. The observations show that there is a great complexity in the susceptibility of nervous tissues to radiotherapy with respect to influences on the expression of neuropeptides.
众所周知，大量因素可影响神经肽在神经系统中的表达。在本研究中，于治疗后十天和六个月检查了对大鼠头颈部进行单侧和双侧照射对支配下颌下腺的神经肽表达以及下颌下神经节神经节细胞中神经肽表达的影响。使用了针对脑啡肽和蛙皮素的抗血清以及免疫组织化学方法。还研究了双侧照射对颈脊髓中各种神经肽染色模式的影响。在双侧治疗十天后，在下颌下腺和下颌下神经节细胞中，两种抗血清染色后均可见神经肽表达明显增加，而单侧治疗后未发生变化。治疗六个月后，下颌下腺/神经节中的神经肽表达模式与对照组相似。照射未导致脊髓中神经肽染色模式发生任何变化。这些观察结果表明，就对神经肽表达的影响而言，神经组织对放射治疗的敏感性存在很大的复杂性。
