Louwagie J, Janssens W, Mascola J, Heyndrickx L, Hegerich P, van der Groen G, McCutchan F E, Burke D S
Henry M. Jackson Foundation Research Laboratory, Rockville, Maryland.
J Virol. 1995 Jan;69(1):263-71. doi: 10.1128/JVI.69.1.263-271.1995.
The genetic diversity of the envelope glycoprotein of human immunodeficiency virus type 1 (HIV-1) isolates was studied. HIV-1 isolates were obtained from eight countries in Africa: Djibouti, Gabon, Kenya, Senegal, Somalia, Uganda, Zaire, and Zambia. The DNA sequences encoding the complete HIV-1 envelope protein were PCR amplified and sequenced. Phylogenetic relationships among the 21 sequences from this study and the 32 previously published full-length env HIV-1 sequences were determined. Twenty of the newly sequenced African isolates could be assigned to envelope subtypes A, C, D, and G. One isolate, collected in Zambia, did not belong to any of the eight previously described subtypes and may represent a prototype sequence of its envelope subtype. The phylogenetic classification of these isolates was strongly supported by bootstrapping and the congruence of trees generated by either distance methods or maximum parsimony analysis. The data presented in this study confirm the existence of several genetic subtypes within the global HIV epidemic and broaden the genetic variability previously observed for envelope subtypes. The geographic spread of different subtypes was shown to be substantial, and the notion of cocirculation of subtypes was reinforced.
对1型人类免疫缺陷病毒（HIV-1）分离株包膜糖蛋白的基因多样性进行了研究。HIV-1分离株来自非洲的八个国家：吉布提、加蓬、肯尼亚、塞内加尔、索马里、乌干达、扎伊尔和赞比亚。对编码完整HIV-1包膜蛋白的DNA序列进行PCR扩增并测序。确定了本研究中的21个序列与之前发表的32个全长env HIV-1序列之间的系统发育关系。新测序的非洲分离株中有20个可归为包膜亚型A、C、D和G。在赞比亚收集的一个分离株不属于之前描述的八个亚型中的任何一个，可能代表其包膜亚型的一个原型序列。通过自展法以及距离法或最大简约分析生成的树的一致性，有力支持了这些分离株的系统发育分类。本研究提供的数据证实了全球HIV流行中存在几种基因亚型，并拓宽了之前观察到的包膜亚型的基因变异性。不同亚型的地理传播范围很大，亚型共同流行的概念得到了强化。
