爱泼斯坦-巴尔病毒核蛋白2酸性结构域可与TFIIB、TAF40和RPA70相互作用，但不与TATA结合蛋白相互作用。
The Epstein-Barr virus nuclear protein 2 acidic domain can interact with TFIIB, TAF40, and RPA70 but not with TATA-binding protein.
作者信息
Tong X, Wang F, Thut C J, Kieff E
机构信息
Department of Microbiology, Harvard University, Boston, Massachusetts 02115.
出版信息
J Virol. 1995 Jan;69(1):585-8. doi: 10.1128/JVI.69.1.585-588.1995.
Abstract
The Epstein-Barr virus nuclear antigen 2 (EBNA-2) acidic domain is essential for B-lymphocyte growth transformation and can activate transcription when brought to a promoter by a sequence-specific DNA-binding domain. We now show that the EBNA-2 acidic domain has slightly less activity than the proteotypic acidic transactivator VP16 in depleting nuclear extracts of basal transcription activity. Like VP16, EBNA-2 associates with TFIIB, TAF40, and RPA70. However, EBNA-2 has much less avidity for TATA-binding protein. A Trp-to-Thr mutation within the acidic domain abolishes EBNA-2 transactivating activity and greatly compromises the association with TFIIB, TAF40, and RPA70, establishing a genetic linkage between transactivating activity and these associations.
摘要
爱泼斯坦-巴尔病毒核抗原2（EBNA-2）酸性结构域对于B淋巴细胞生长转化至关重要，当通过序列特异性DNA结合结构域被带到启动子时，它能够激活转录。我们现在表明，EBNA-2酸性结构域在消耗基础转录活性的核提取物方面，其活性略低于典型酸性反式激活因子VP16。与VP16一样，EBNA-2与TFIIB、TAF40和RPA70相关联。然而，EBNA-2与TATA结合蛋白的亲和力要低得多。酸性结构域内的色氨酸到苏氨酸突变消除了EBNA-2反式激活活性，并极大地损害了与TFIIB、TAF40和RPA70的关联，从而在反式激活活性与这些关联之间建立了遗传联系。
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The transactivator proteins VP16 and GAL4 bind replication factor A.反式激活蛋白VP16和GAL4与复制因子A结合。
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The Epstein-Barr virus immortalizing protein EBNA-2 is targeted to DNA by a cellular enhancer-binding protein.爱泼斯坦-巴尔病毒永生化蛋白EBNA-2通过一种细胞增强子结合蛋白靶向DNA。
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Cell. 1993 Nov 5;75(3):519-30. doi: 10.1016/0092-8674(93)90386-5.
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The EBNA-2 arginine-glycine domain is critical but not essential for B-lymphocyte growth transformation; the rest of region 3 lacks essential interactive domains.EBNA - 2的精氨酸 - 甘氨酸结构域对B淋巴细胞生长转化至关重要，但并非必不可少；区域3的其余部分缺乏关键的相互作用结构域。
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The Epstein-Barr virus nuclear antigen 2 transactivator is directed to response elements by the J kappa recombination signal binding protein.爱泼斯坦-巴尔病毒核抗原2反式激活因子通过Jκ重组信号结合蛋白被导向反应元件。
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