Nakayama T, Nakabayashi H
2nd Department of Internal Medicine.
Nihon Rinsho. 1994 Oct;52(10):2720-5.
Using PCR-RFLP analysis, no A2 allele which is related with the frequency of NIDDM in Finland was detected in Japanese patients with NIDDMs and controls. We found out a new microsatellite of CA repeat in the human muscle glycogen synthase (MGS) gene, and the polymorphism of the repeat number detected by the PCR. The allele frequency was not significantly different between NIDDMs and controls. However, the allele frequency in the patients without high total cholesterolemia was significantly different from that of controls, and 19 repeat allele was significantly higher in this group than that in controls. These suggest that the MGS gene or neighboring genes are related with one of the disease genes of diabetes mellitus without high total cholesterolemia.
通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析，在日本2型糖尿病患者及对照组中未检测到与芬兰2型糖尿病发病频率相关的A2等位基因。我们发现了人类肌肉糖原合酶（MGS）基因中一个新的CA重复微卫星序列，并通过PCR检测了该重复序列数目的多态性。2型糖尿病患者与对照组之间的等位基因频率无显著差异。然而，无高总胆固醇血症患者的等位基因频率与对照组显著不同，该组中19次重复等位基因显著高于对照组。这些结果提示，MGS基因或其邻近基因与无高总胆固醇血症的糖尿病致病基因之一相关。
