di Filippo A, Scardi S, Consalvo M, Ridolfi N, Pellegrini G, Paternostro E, Novelli G P
Istituto di Anestesia e Rianimazione, Università degli Studi di Firenze.
Minerva Anestesiol. 1994 Jun;60(6):295-303.
BACKGROUND AND WORK HYPOTHESIS: The mechanisms of organ damage in MODS are unclear. Given that lipid peroxidation induced by oxygen radicals may play a key role in organ damage, expired ethane, a marker of lipid peroxidation, could be correlated to the entity and evolution of experimental MODS.
Forty wistar male rats (250 +/- 15 g body weight) were intraperitoneally injected with 10 ml of Zymosan mineral oil suspension (2.5%) to provoke MODS. Survival and symptoms were noted daily. On the 2nd, 7th and 14th day the animals underwent thorax CT scanning. CT images were processed to evaluate the relative density of lung parenchima. Expired ethane was measured using mass spectrometer and percentual changes were noted. Relative organ weight (liver and lung) were measured in 2nd, 7th and 14th day.
Pearson's linear correlation.
The results show that maximal lung damage took place at the same moments as ethane concentrations peaked. A strict correlation (r = 0.93; p < 0.0001) was found between lung density and expired ethane. Strict correlation was found between organ weight, symptoms, survival and ethane production.
Lipid peroxidation, as expressed by ethane production, might be an important cause of organ damage in MODS.
背景与工作假设：多器官功能障碍综合征（MODS）中器官损伤的机制尚不清楚。鉴于氧自由基诱导的脂质过氧化可能在器官损伤中起关键作用，呼出乙烷作为脂质过氧化的一个标志物，可能与实验性MODS的发生及发展相关。
40只雄性Wistar大鼠（体重250±15克）腹腔注射10毫升酵母聚糖矿物油悬液（2.5%）以诱发MODS。每天记录存活情况和症状。在第2、7和14天对动物进行胸部CT扫描。对CT图像进行处理以评估肺实质的相对密度。使用质谱仪测量呼出乙烷，并记录其百分比变化。在第2、7和14天测量相对器官重量（肝脏和肺）。
Pearson线性相关性分析。
结果显示，肺部最大损伤发生在乙烷浓度达到峰值的同一时刻。肺密度与呼出乙烷之间存在密切相关性（r = 0.93；p < 0.0001）。在器官重量、症状、存活情况与乙烷产生之间也发现了密切相关性。
以乙烷产生所表示的脂质过氧化可能是MODS中器官损伤的一个重要原因。
