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A rapid, reliable method for detection of known point mutations: point-EXACCT.

作者信息

Somers V A, Moerkerk P T, Murtagh J J, Thunnissen F B

机构信息

Department of Pathology, University of Limburg, Maastricht, The Netherlands.

出版信息

Nucleic Acids Res. 1994 Nov 11;22(22):4840-1. doi: 10.1093/nar/22.22.4840.

DOI:10.1093/nar/22.22.4840
PMID:7984438
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC308539/
Abstract
摘要

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引用本文的文献

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Sensitive and specific detection of K-ras mutations in colon tumors by short oligonucleotide mass analysis.通过短寡核苷酸质量分析对结肠肿瘤中K-ras突变进行灵敏且特异的检测。
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本文引用的文献

1
An exonuclease-amplification coupled capture technique improves detection of PCR product.一种核酸外切酶扩增偶联捕获技术可提高PCR产物的检测效率。
Nucleic Acids Res. 1993 Aug 11;21(16):3905-6. doi: 10.1093/nar/21.16.3905.
2
Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations.两种不同的点突变导致人结肠癌和肺癌中Ki-ras2基因的激活。
Nature. 1983;304(5926):507-13. doi: 10.1038/304507a0.
3
Four human carcinoma cell lines with novel mutations in position 12 of c-K-ras oncogene.四种在c-K-ras癌基因第12位存在新突变的人类癌细胞系。
Nucleic Acids Res. 1986 Jan 24;14(2):843-52. doi: 10.1093/nar/14.2.843.
4
The molecular genetics of cancer.癌症的分子遗传学
Science. 1987 Jan 16;235(4786):305-11. doi: 10.1126/science.3541204.
5
Disease diagnosis by recombinant DNA methods.采用重组DNA方法进行疾病诊断。
Science. 1987 Jun 5;236(4806):1223-9. doi: 10.1126/science.3296189.
6
The ras gene family and human carcinogenesis.Ras基因家族与人类致癌作用。
Mutat Res. 1988 May;195(3):255-71. doi: 10.1016/0165-1110(88)90004-8.
7
DNA diagnostics--molecular techniques and automation.DNA诊断——分子技术与自动化
Science. 1988 Oct 14;242(4876):229-37. doi: 10.1126/science.3051381.
8
Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。
Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.
9
PCR amplification of specific alleles (PASA) is a general method for rapidly detecting known single-base changes.特定等位基因的聚合酶链反应扩增(PASA)是一种快速检测已知单碱基变化的通用方法。
Biotechniques. 1992 Jan;12(1):82-7.