一种核酸外切酶扩增偶联捕获技术可提高PCR产物的检测效率。 - Suppr

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超能文献

An exonuclease-amplification coupled capture technique improves detection of PCR product.

作者信息

Holloway B, Erdman D D, Durigon E L, Murtagh J J

机构信息

Centers for Disease Control and Prevention, National Center for Infectious Diseases, Atlanta, GA.

出版信息

Nucleic Acids Res. 1993 Aug 11;21(16):3905-6. doi: 10.1093/nar/21.16.3905.

DOI:10.1093/nar/21.16.3905

PMID:8396241

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC309932/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/05d2/309932/018ce2738536/nar00065-0295-a.jpg

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本文引用的文献

Gene 6 exonuclease of bacteriophage T7. II. Mechanism of the reaction.噬菌体T7的基因6核酸外切酶。II. 反应机制。

J Biol Chem. 1972 Jan 10;247(1):311-8.

Generation of single-stranded DNA by the polymerase chain reaction and its application to direct sequencing of the HLA-DQA locus.通过聚合酶链反应生成单链DNA及其在HLA - DQA基因座直接测序中的应用。

Proc Natl Acad Sci U S A. 1988 Oct;85(20):7652-6. doi: 10.1073/pnas.85.20.7652.

Physicochemical properties of phosphorothioate oligodeoxynucleotides.硫代磷酸酯寡脱氧核苷酸的物理化学性质。

Nucleic Acids Res. 1988 Apr 25;16(8):3209-21. doi: 10.1093/nar/16.8.3209.

Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase.使用热稳定DNA聚合酶进行引物引导的DNA酶促扩增。

Science. 1988 Jan 29;239(4839):487-91. doi: 10.1126/science.2448875.

DNA enzyme immunoassay: general method for detecting products of polymerase chain reaction.

Clin Chem. 1991 Mar;37(3):422-9.

Polymerase chain reaction: trenches to benches.聚合酶链反应：从战壕到实验台。

J Clin Microbiol. 1991 Jul;29(7):1281-5. doi: 10.1128/jcm.29.7.1281-1285.1991.

Single-base mutational analysis of cancer and genetic diseases using membrane bound modified oligonucleotides.使用膜结合修饰寡核苷酸对癌症和遗传疾病进行单碱基突变分析。

Nucleic Acids Res. 1991 Jul 25;19(14):3929-33. doi: 10.1093/nar/19.14.3929.

In vitro production of large single-stranded templates for DNA sequencing.用于DNA测序的大的单链模板的体外制备。

Biotechniques. 1991 Mar;10(3):376-84.

The polymerase chain reaction: clinical applications.聚合酶链反应：临床应用

Adv Clin Chem. 1992;29:161-96. doi: 10.1016/s0065-2423(08)60224-3.

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