Keane T E, Petros J A, Velimirovich B, Yue K T, Graham S D
Department of Surgery, Emory University School of Medicine, Atlanta, Georgia.
Urology. 1994 Dec;44(6):842-6. doi: 10.1016/s0090-4295(94)80168-1.
The goal of this research was to assess whether methoxypsoralen compounds in combination with ultraviolet light were effective in preventing cellular proliferation in an in vitro model of human transitional cell carcinoma.
Three methoxypsoralen compounds, 5-methoxypsoralen (5-MOP), 8-methoxypsoralen (8-MOP), and 4'-aminomethyl 4,5'-8'-trimethylpsoralen (AMT), were added in vitro to T-24 transitional cell carcinoma cells. Psoralens directly bind to DNA, cross-linking the strands when exposed to ultraviolet light and thereby prevent cellular division.
In vitro activity was demonstrated utilizing AMT and ultraviolet radiation at 320 to 340 nm, preventing cellular proliferation in T-24 transitional cell carcinoma.
Methoxypsoralen compounds in combination with ultraviolet light are effective in preventing proliferation of bladder carcinoma cells in vitro. This therapy may prove to be effective in clinical early stage transitional cell carcinoma and warrants further assessment.
本研究的目的是评估甲氧补骨脂素化合物与紫外线联合使用在人移行细胞癌体外模型中预防细胞增殖是否有效。
将三种甲氧补骨脂素化合物，即5-甲氧补骨脂素（5-MOP）、8-甲氧补骨脂素（8-MOP）和4'-氨甲基-4,5'-8'-三甲基补骨脂素（AMT），体外添加到T-24移行细胞癌细胞中。补骨脂素直接与DNA结合，在暴露于紫外线时使链交联，从而防止细胞分裂。
利用AMT和320至340nm的紫外线辐射证明了体外活性，可防止T-24移行细胞癌细胞的细胞增殖。
甲氧补骨脂素化合物与紫外线联合使用在体外可有效预防膀胱癌细胞增殖。这种疗法可能在临床早期移行细胞癌中被证明是有效的，值得进一步评估。
