Tak P P, Kummer J A, Hack C E, Daha M R, Smeets T J, Erkelens G W, Meinders A E, Kluin P M, Breedveld F C
University Hospital of Leiden, The Netherlands.
Arthritis Rheum. 1994 Dec;37(12):1735-43. doi: 10.1002/art.1780371205.
To define the expression and the phenotype of granzyme (Gran) A and B positive cytotoxic cells in synovial tissue (ST) from patients with rheumatoid arthritis (RA) with respect to disease duration and activity.
Using antibodies against GranA and GranB, which serve as markers of activated natural killer (NK) cells and cytotoxic T lymphocytes, ST sections from 10 patients with early RA, 10 patients with longstanding RA, and 10 patients with osteoarthritis were examined. The phenotype of Gran+ cells was determined with double-labeling techniques.
Gran+ cells, the majority of which were NK cells, were found in ST from patients in all groups. Several of these cells did not express the surface markers CD16, CD56, and CD57. The highest ST expression of GranB was found in patients with early RA. In RA patients, there was a positive correlation of GranB expression with serum levels of acute-phase reactants, but not with histologic scores for inflammation.
Gran+ cells are mainly NK cells, a substantial proportion of which do not express conventional NK cell surface markers. GranB expression is specifically increased in the synovial tissues of patients with RA of short duration.
明确类风湿关节炎（RA）患者滑膜组织（ST）中颗粒酶（Gran）A和B阳性细胞毒性细胞的表达及表型与疾病病程和活动度的关系。
使用抗GranA和GranB抗体（作为活化自然杀伤（NK）细胞和细胞毒性T淋巴细胞的标志物），对10例早期RA患者、10例长期RA患者和10例骨关节炎患者的ST切片进行检测。采用双标记技术确定Gran+细胞的表型。
所有组患者的ST中均发现Gran+细胞，其中大多数为NK细胞。这些细胞中有几个不表达表面标志物CD16、CD56和CD57。GranB在早期RA患者的ST中表达最高。在RA患者中，GranB表达与急性期反应物血清水平呈正相关，但与炎症组织学评分无关。
Gran+细胞主要是NK细胞，其中很大一部分不表达传统的NK细胞表面标志物。GranB表达在病程较短的RA患者滑膜组织中特异性增加。
