Robinson M S
University of Cambridge, Department of Clinical Biochemistry, Addenbrooke's Hospital, UK.
Curr Opin Cell Biol. 1994 Aug;6(4):538-44. doi: 10.1016/0955-0674(94)90074-4.
Clathrin-coated vesicles bud from the plasma membrane and from the trans-Golgi network; both populations of coated vesicles participate in the endocytic pathway. Coated-vesicle formation is initiated by the binding of cytosolic adaptor complexes to putative adaptor receptors on the appropriate membrane. Clathrin then binds to the adaptors and assembles to form a coated bud, which pinches off as a coated vesicle. The GTPase dynamin facilitates the formation of coated vesicles at the plasma membrane through an as yet unknown mechanism. Recent studies suggest that regulatory mechanisms may operate at each of these stages.
网格蛋白包被小泡从质膜和反式高尔基体网络出芽；这两类包被小泡都参与内吞途径。包被小泡的形成始于胞质衔接蛋白复合物与合适膜上假定的衔接蛋白受体的结合。然后网格蛋白与衔接蛋白结合并组装形成包被芽，包被芽作为包被小泡脱离。GTP酶发动蛋白通过一种尚不清楚的机制促进质膜处包被小泡的形成。最近的研究表明，调控机制可能在这些阶段的每一个环节发挥作用。
