将“红色丙酸杆菌”重新分类为詹氏丙酸杆菌。

Reclassification of "Propionibacterium rubrum" as P. jensenii.

作者信息

de Carvalho A F, Guezenec S, Gautier M, Grimont P A

机构信息

Laboratoire de Recherches de Technologie laitière, INRA, Rennes, France.

出版信息

Res Microbiol. 1995 Jan;146(1):51-8. doi: 10.1016/0923-2508(96)80270-1.

DOI:10.1016/0923-2508(96)80270-1

PMID:7754229

Abstract

The taxonomic relationship of strains previously designated as "Propionibacterium rubrum" to P. thoenii and P. jensenii was investigated by use of 16S ribosomal RNA sequence comparison, biochemical characteristics and DNA hybridization. A total of 46 strains representing the species P. jensenii and P. thoenii and the former species "P. rubrum" and also including 21 reference strains and 25 strains isolated from dairy sources were studied. The 16S rRNA sequence of strain "P. rubrum" CNRZ 85 (= ATCC 4871) was found to be almost identical to that of the type strain of P. jensenii. DNA hybridization data indicated that "P. rubrum" should belong to the species P. jensenii rather than P. thoenii, as formerly proposed. The "P. rubrum" strains should then be reclassified as a beta-haemolytic biovar of P. jensenii. The genomic species P. jensenii and P. thoenii could be differentiated by biochemical characteristics such as the production of acid from myo-inositol and starch.

摘要

通过16S核糖体RNA序列比较、生化特性及DNA杂交技术，研究了先前被指定为“红色丙酸杆菌”的菌株与托氏丙酸杆菌和詹氏丙酸杆菌之间的分类关系。共研究了46株代表詹氏丙酸杆菌、托氏丙酸杆菌以及先前的“红色丙酸杆菌”物种的菌株，其中包括21株参考菌株和25株从乳制品中分离出的菌株。发现菌株“红色丙酸杆菌”CNRZ 85（=ATCC 4871）的16S rRNA序列与詹氏丙酸杆菌模式菌株的序列几乎相同。DNA杂交数据表明，“红色丙酸杆菌”应属于詹氏丙酸杆菌物种，而非先前提出的托氏丙酸杆菌。因此，“红色丙酸杆菌”菌株应重新分类为詹氏丙酸杆菌的β-溶血生物变种。詹氏丙酸杆菌和托氏丙酸杆菌这两个基因组物种可通过生化特性（如从肌醇和淀粉产酸）来区分。