Tsujiuchi T, Tsutsumi M, Honoki K, Horiguchi K, Kobayashi E, Denda A, Konishi Y
Department of Oncological Pathology, Cancer Center, Nara Medical College, Kashihara, Japan.
Exp Toxicol Pathol. 1994 Jul;46(2):115-8. doi: 10.1016/s0940-2993(11)80037-0.
Effects of novobiocin on the induction of gamma-glutamyltranspeptidase(GGT)-positive foci, an early lesion occurring during hepatocarcinogenesis, after diethylnitrosamine(DEN) initiation were investigated in Fischer 344 rats. Animals were given DEN at a dose of 20 mg/kg b. w. followed by novobiocin at doses of 50, 100 and 200 mg/kg b. w. The latter two doses, but not 50 mg/kg b. w., significantly inhibited the development of GGT-positive foci, providing evidence of the possible involvement of mono(ADP-ribosyl)ation in the initiation phase of hepatocarcinogenesis in rats.
在Fischer 344大鼠中研究了新生霉素对二乙基亚硝胺（DEN）启动后γ-谷氨酰转肽酶（GGT）阳性灶诱导的影响，GGT阳性灶是肝癌发生过程中出现的早期病变。给动物注射剂量为20mg/kg体重的DEN，随后分别注射剂量为50、100和200mg/kg体重的新生霉素。后两个剂量（而非50mg/kg体重）显著抑制了GGT阳性灶的发展，这为单（ADP-核糖基）化可能参与大鼠肝癌发生起始阶段提供了证据。
