Brown K E, Barnett M A, Burgtorf C, Shaw P, Buckle V J, Brown W R
Biochemistry Department, Oxford University, UK.
Hum Mol Genet. 1994 Aug;3(8):1227-37. doi: 10.1093/hmg/3.8.1227.
We have used telomeric DNA to break the human Y chromosome within the centromeric array of alphoid satellite DNA and have created two derivative chromosomes; one consists of the short arm and 140 kb of alphoid DNA, the other consists of the long arm and 480 kb of alphoid DNA. Both segregate accurately at mitosis. It is known that there is no large scale sequence duplication around the alphoid DNA and so the simplest interpretation of our results is that the sequence responsible for accurate segregation is the alphoid DNA itself. Although the long arm acrocentric derivative segregates accurately it lags with respect to the other chromosomes in about 10% of anaphase cells and thus additional sequences may be required for orderly segregation. The short arm acrocentric chromosome is probably no larger than 12 Mb in size and thus our results also demonstrate that chromosomes of this size are capable of accurate segregation.
我们利用端粒DNA在α卫星DNA的着丝粒阵列内断裂人类Y染色体，并创建了两条衍生染色体；一条由短臂和140 kb的α卫星DNA组成，另一条由长臂和480 kb的α卫星DNA组成。两者在有丝分裂时都能准确分离。已知在α卫星DNA周围没有大规模的序列重复，因此对我们结果的最简单解释是，负责精确分离的序列就是α卫星DNA本身。尽管长臂近端着丝粒衍生染色体能准确分离，但在约10%的后期细胞中，它相对于其他染色体滞后，因此有序分离可能还需要其他序列。短臂近端着丝粒染色体大小可能不超过12 Mb，因此我们的结果也表明，这种大小的染色体能够精确分离。
