Engelmann M, Ludwig M, Landgraft R
Max Planck Institute of Psychiatry, Clinical Institute, Munich, Germany.
J Neuroendocrinol. 1994 Aug;6(4):391-5. doi: 10.1111/j.1365-2826.1994.tb00598.x.
We previously reported that direct osmotic stimulation of the supraoptic nucleus (SON) of rats via a microdialysis probe produces a robust 'rebound' release of endogenous vasopressin (AVP) into the extracellular fluid of this hypothalamic nucleus. We now demonstrate in a combined microdialysis and push-pull perfusion study that this intranuclear release is accompanied by increased AVP release in the septum. Simultaneous monitoring of intranuclear release and behavioral performance in the same animal indicated a significant correlation between the amount of endogenously released AVP and improved social memory based on the olfactory discriminative capacities of adult male rats. This memory improvement was partially blocked by local administration of a AVP V1 receptor antagonist either into the SON or septum. These findings indicate that direct osmotic stimulation of the supraoptic nucleus, which increases intracerebral vasopressin release, improves the acquisition and/or processing of olfactory stimuli. Thus, the endogenous neuropeptide fulfills one of the major criteria for being causally involved as a neurotransmitter/neuromodulator in behavioral performance.
我们先前报道，通过微透析探针直接对大鼠视上核（SON）进行渗透刺激，会使内源性血管加压素（AVP）大量“反弹”释放到该下丘脑核的细胞外液中。我们现在在一项微透析和推挽灌注联合研究中证明，这种核内释放伴随着隔区AVP释放增加。在同一动物中同时监测核内释放和行为表现表明，基于成年雄性大鼠的嗅觉辨别能力，内源性释放的AVP量与改善的社会记忆之间存在显著相关性。向视上核或隔区局部注射AVP V1受体拮抗剂可部分阻断这种记忆改善。这些发现表明，对视上核的直接渗透刺激增加了脑内血管加压素的释放，改善了嗅觉刺激的获取和/或处理。因此，内源性神经肽符合作为神经递质/神经调节剂因果参与行为表现的主要标准之一。
