Larramendy M L, Björkqvist A M, Tammilehto L, Taavitsainen M, Mattson K, Knuutila S
Department of Medical Genetics, University of Helsinki, Finland.
Cancer Genet Cytogenet. 1994 Nov;78(1):78-81. doi: 10.1016/0165-4608(94)90050-7.
Trisomy 7 is a frequent aneuploid change in lymphomas, adenocarcinomas, and malignant mesenchymal and neurogenic tumors. Moreover, it has been observed in cultured and uncultured non-neoplastic cells from brain, kidney, liver, lung, and atherosclerotic plaques, among other tissues, opening debate on the role of this change in normal and neoplastic tissue. We used nonradioactive in situ hybridization (ISH) with a biotinylated chromosome 7-specific alpha-satellite DNA probe to seek an extra copy of chromosome 7 in ascitic and pleural fluid interphase cells from 26 donors. The donors comprised 24 patients with nonmalignant clinical history, one patient with non-Hodgkin's malignant lymphoma (positive control), and one patient with chronic myeloid leukemia (CML, negative control). The highest frequency of fluid cells with three hybridization signals in patients without neoplasia was 0.5%, in contrast to the frequency of 40.5% noted in the fluid cells of the patient with non-Hodgkin's malignant lymphoma. The results demonstrate that the frequency of trisomic cells in pleural as well as in ascitic fluid is very low, making possible use of the cells in ascitic or pleural fluids in identification of malignancy.
7号染色体三体是淋巴瘤、腺癌以及恶性间叶组织和神经源性肿瘤中常见的非整倍体改变。此外，在来自脑、肾、肝、肺以及动脉粥样硬化斑块等组织的培养和未培养的非肿瘤细胞中也观察到了这种情况，这引发了关于这种改变在正常组织和肿瘤组织中作用的争论。我们使用生物素化的7号染色体特异性α-卫星DNA探针进行非放射性原位杂交（ISH），以在26名供体的腹水和胸水间期细胞中寻找额外的7号染色体拷贝。供体包括24名有非恶性临床病史的患者、1名非霍奇金恶性淋巴瘤患者（阳性对照）和1名慢性粒细胞白血病患者（CML，阴性对照）。无肿瘤患者中具有三个杂交信号的体液细胞的最高频率为0.5%，相比之下，非霍奇金恶性淋巴瘤患者的体液细胞中该频率为40.5%。结果表明，胸水和腹水中三体细胞的频率非常低，这使得利用腹水或胸水中的细胞来鉴定恶性肿瘤成为可能。
