Tallis G A, Shephard M D, Whiting M J
Department of Biochemistry and Chemical Pathology, Flinders Medical Centre, Bedford Park, Australia.
Clin Chim Acta. 1994 Aug;228(2):171-9. doi: 10.1016/0009-8981(94)90287-9.
High performance gel chromatography (HPGC) was used to separate lipoproteins on the basis of their size and to generate lipoprotein profiles for plasma collected from patients with different lipoprotein phenotypes. These profiles provided a direct measurement of low density lipoprotein (LDL)-cholesterol which was more precise than LDL-cholesterol values calculated by the Friedewald equation. In addition, LDL-cholesterol concentrations were obtained in patients with combined hyperlipidemia in whom LDL-cholesterol could not be accurately calculated by the Friedewald equation. The response of LDL-cholesterol to the drug gemfibrozil was reliably monitored and in addition changes in LDL particle size could be assessed from the LDL apolipoprotein B/cholesterol ratio. HPGC also assisted in the diagnosis of type III hyperlipidemia by revealing a characteristic lipoprotein profile. HPGC-derived lipoprotein profiles provided additional useful clinical information for combined hyperlipidemia (Fredrickson lipoprotein phenotypes IIb, III).
高效凝胶色谱法（HPGC）用于根据脂蛋白大小分离脂蛋白，并为不同脂蛋白表型患者采集的血浆生成脂蛋白谱。这些谱图提供了低密度脂蛋白（LDL）胆固醇的直接测量值，该值比通过Friedewald方程计算的LDL胆固醇值更精确。此外，对于合并高脂血症且无法通过Friedewald方程准确计算LDL胆固醇的患者，也能获得其LDL胆固醇浓度。可靠地监测了LDL胆固醇对吉非贝齐药物的反应，此外，还可根据LDL载脂蛋白B/胆固醇比值评估LDL颗粒大小的变化。HPGC还通过揭示特征性脂蛋白谱辅助诊断III型高脂血症。HPGC衍生的脂蛋白谱为合并高脂血症（Fredrickson脂蛋白表型IIb、III）提供了额外有用的临床信息。
