细胞色素P-450 3A在HT29-MTX细胞和Caco-2克隆TC7中的表达。
Expression of cytochrome P-450 3A in HT29-MTX cells and Caco-2 clone TC7.
作者信息
Carrière V, Lesuffleur T, Barbat A, Rousset M, Dussaulx E, Costet P, de Waziers I, Beaune P, Zweibaum A
机构信息
Unité de Recherches de Biochimie Pharmacologique et Métabolique (INSERM U75), Centre Hospitalo-Universitaire Necker-Enfants Malades, Paris, France.
出版信息
FEBS Lett. 1994 Dec 5;355(3):247-50. doi: 10.1016/0014-5793(94)01199-0.
HT-29 sublines and Caco-2 clones were analyzed for the expression of cytochrome P-450 3A. The enzyme was found to be expressed in differentiated HT-29 cells selected by resistance to methotrexate and in one of seven Caco-2 clones, TC7. Its expression parallels the differentiation process, with highest levels being observed at late confluency. P-450 3A mRNA and protein patterns, as well as subcellular distribution, are intermediate between those observed in human adult intestine and fetal liver.
对HT - 29亚系和Caco - 2克隆进行细胞色素P - 450 3A表达分析。发现该酶在通过对甲氨蝶呤耐药筛选出的分化HT - 29细胞以及七个Caco - 2克隆之一的TC7中表达。其表达与分化过程平行，在汇合后期观察到最高水平。P - 450 3A的mRNA和蛋白质模式以及亚细胞分布介于人类成人肠道和胎儿肝脏中观察到的模式之间。
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