Takahashi M, Tezuka T, Katunuma N
Department of Dermatology, Kinki University School of Medicine, Osaka, Japan.
FEBS Lett. 1994 Dec 5;355(3):275-8. doi: 10.1016/0014-5793(94)01196-6.
The activity of a cysteine proteinase purified from Staphylococcus aureus V8 (SAV8) was inhibited by phosphorylated cystatin alpha (P-cystatin alpha) and by purified cornified envelope protein of newborn rat, a conjugated form of P-cystatin alpha. Immunohistochemical analysis demonstrated a marked decrease in P-cystatin alpha content in cornified envelope treated with sphingosine. The inhibition of papain activity by proteins from sphingosine-treated skin was much weaker than that exerted by proteins from the untreated skin. The suppression of SAV8 colony formation inoculated on the sphingosine-treated skin was examined. Colony formation on the sphingosine-treated skin was enhanced compared to that on normal skin. These findings suggest that P-cystatin alpha in the cornified envelope may have a bacteriostatic barrier function against bacterial infection, such as that with SAV8.
从金黄色葡萄球菌V8（SAV8）中纯化出的半胱氨酸蛋白酶的活性，受到磷酸化的胱抑素α（P-胱抑素α）以及新生大鼠纯化的角质包膜蛋白（P-胱抑素α的一种共轭形式）的抑制。免疫组织化学分析表明，经鞘氨醇处理的角质包膜中P-胱抑素α含量显著降低。鞘氨醇处理过的皮肤中的蛋白质对木瓜蛋白酶活性的抑制作用，比未处理皮肤中的蛋白质所产生的抑制作用弱得多。研究了接种在经鞘氨醇处理的皮肤上的SAV8菌落形成情况。与正常皮肤相比，经鞘氨醇处理的皮肤上的菌落形成有所增强。这些发现表明，角质包膜中的P-胱抑素α可能对诸如SAV8引起的细菌感染具有抑菌屏障功能。
