Blankenvoorde M F, van't Hof W, Walgreen-Weterings E, van Steenbergen T J, Brand H S, Veerman E C, Nieuw Amerongen A V
Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), The Netherlands.
Biol Chem. 1998 Nov;379(11):1371-5.
We investigated whether cystatins and cystatin-derived peptides, encompassing sequences of secondary structures of cystatin S and papain binding domains of cystatin C, display antimicrobial properties. Of the different microorganisms tested, only the growth of P. gingivalis was inhibited by chicken cystatin and cystatin C. Cystatin S, cystatin S:1-14, cystatin S:61-73 and cystatin S:108-121 also inhibited its growth, whereas cystatin S:21-38, cystatin S:39-55, cystatin S:81-95, cystatin S:94-109, and cystatin C: 9-12/55-60/106-107 did not. No inhibition of the cysteine proteinase activity of P. gingivalis was observed for all cystatin-derived peptides. On the other hand, leupeptin and antipain inhibited P. gingivalis proteinase activity, but had no effect on the growth. These data suggest that cystatins contain antibacterial sequences active against P. gingivalis and that the growth inhibition does not depend on the inhibition of P. gingivalis cysteine proteinases.
我们研究了包括胱抑素S二级结构序列和胱抑素C木瓜蛋白酶结合域的胱抑素及胱抑素衍生肽是否具有抗菌特性。在测试的不同微生物中，只有牙龈卟啉单胞菌的生长受到鸡胱抑素和胱抑素C的抑制。胱抑素S、胱抑素S:1 - 14、胱抑素S:61 - 73和胱抑素S:108 - 121也抑制其生长，而胱抑素S:21 - 38、胱抑素S:39 - 55、胱抑素S:81 - 95、胱抑素S:94 - 109以及胱抑素C: 9 - 12/55 - 60/106 - 107则没有这种作用。所有胱抑素衍生肽均未观察到对牙龈卟啉单胞菌半胱氨酸蛋白酶活性的抑制作用。另一方面，亮抑酶肽和抗蛋白酶抑制牙龈卟啉单胞菌蛋白酶活性，但对其生长没有影响。这些数据表明，胱抑素含有对牙龈卟啉单胞菌有活性的抗菌序列，且生长抑制并不依赖于对牙龈卟啉单胞菌半胱氨酸蛋白酶的抑制。
