McClelland R G, Pinder A C
Department of Food Biophysics, AFRC Institute of Food Research, Norwich Laboratory, UK.
J Appl Bacteriol. 1994 Oct;77(4):440-7. doi: 10.1111/j.1365-2672.1994.tb03447.x.
The use of fluorescently-labelled monoclonal antibodies, with detection by multi-parameter flow cytometry, was investigated for the rapid detection of salmonellas in pure cultures. Accurate detection of specific Salmonella serotypes was demonstrated down to levels of below 10(4) cells ml-1 (within 30 min) and 1 cell ml-1 (after 6 h non-selective pre-enrichment). This level of sensitivity was attained even in the presence of high levels of other bacterial species that would otherwise have interfered with the results. With combinations of different antibodies, each with a unique fluorescent label, simultaneous analysis for two species was possible.
研究了使用荧光标记的单克隆抗体并通过多参数流式细胞术进行检测，以快速检测纯培养物中的沙门氏菌。已证明能够准确检测特定沙门氏菌血清型，检测限低至10⁴个细胞/毫升以下（30分钟内）和1个细胞/毫升（6小时非选择性预富集后）。即使存在高水平的其他细菌种类，否则会干扰结果，也能达到这种灵敏度水平。通过组合不同的抗体，每种抗体都有独特的荧光标记，可以同时分析两种细菌。
