McClelland R G, Pinder A C
Food Biophysics Department, Institute of Food Research Norwich Laboratory, Colney, United Kingdom.
Appl Environ Microbiol. 1994 Dec;60(12):4255-62. doi: 10.1128/aem.60.12.4255-4262.1994.
Flow cytometry, combined with fluorescently labelled monoclonal antibodies, offers advantages of speed and sensitivity for the detection of specific pathogenic bacteria in foods. We investigated the detection of Salmonella typhimurium in eggs and milk. Using a sample clearing procedure, we determined that the detection limit was on the order of 10(3) cells per ml after a total analysis time of 40 min. After 6 h of nonselective enrichment, the detection limits were 10 cells per ml for milk and 1 cell per ml for eggs, even in the presence of a 10,000-fold excess of Escherichia coli cells.
流式细胞术结合荧光标记的单克隆抗体，在检测食品中的特定病原菌时具有速度快和灵敏度高的优点。我们研究了鸡蛋和牛奶中鼠伤寒沙门氏菌的检测。采用样品净化程序，我们确定在40分钟的总分析时间后，检测限约为每毫升10³个细胞。经过6小时的非选择性富集后，即使存在10000倍过量的大肠杆菌细胞，牛奶的检测限为每毫升10个细胞，鸡蛋的检测限为每毫升1个细胞。
