食品中鼠伤寒沙门氏菌的酶联免疫吸附测定：1天内检测沙门氏菌的可行性
Enzyme-linked immunosorbent assay for Salmonella typhimurium in food: feasibility of 1-day Salmonella detection.
作者信息
Lee H A, Wyatt G M, Bramham S, Morgan M R
机构信息
AFRC Institute of Food Research, Norwich Laboratory, United Kingdom.
出版信息
Appl Environ Microbiol. 1990 Jun;56(6):1541-6. doi: 10.1128/aem.56.6.1541-1546.1990.
Abstract
A microtitration plate, antibody-capture, enzyme-linked immunosorbent assay was developed for detection of Salmonella typhimurium. The assay utilizes a monoclonal detector antibody which shows no cross-reactions with non-Salmonella species and only a slight cross-reaction with one other Salmonella serotype. By using only one cultural stage (in a nonselective, chemically defined medium) prior to the enzyme-linked immunosorbent assay, low numbers of cells in food (10 cells 25 g-1) were detected in 19 h. Non-Salmonella competing organisms did not interfere with detection of S. typhimurium even when present in the ratio of 10(6):1 (non-Salmonella/Salmonella spp.). The assay shows the feasibility of rapid, 1-day testing for Salmonella spp. with antibody technology.
摘要
开发了一种用于检测鼠伤寒沙门氏菌的微量滴定板抗体捕获酶联免疫吸附测定法。该测定法使用一种单克隆检测抗体，该抗体与非沙门氏菌属无交叉反应，仅与另一种沙门氏菌血清型有轻微交叉反应。通过在酶联免疫吸附测定之前仅使用一个培养阶段（在非选择性化学限定培养基中），在19小时内检测到食品中低数量的细胞（10个细胞/25 g-1）。即使非沙门氏菌竞争菌以10(6):1（非沙门氏菌/沙门氏菌属）的比例存在，也不会干扰鼠伤寒沙门氏菌的检测。该测定法显示了使用抗体技术对沙门氏菌属进行快速一日检测的可行性。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Isolation of members of the genus Salmonella by membrane filter procedures.通过膜过滤法分离沙门氏菌属的成员。
Appl Microbiol. 1957 Sep;5(5):305-7. doi: 10.1128/am.5.5.305-307.1957.
3
Preparation of monoclonal antibodies: strategies and procedures.单克隆抗体的制备：策略与程序
Methods Enzymol. 1981;73(Pt B):3-46. doi: 10.1016/0076-6879(81)73054-4.
4
An enzyme immunoassay for the detection of all Salmonella using a combination of a myeloma protein and a hybridoma antibody.
J Immunol Methods. 1984 Oct 12;73(1):147-56. doi: 10.1016/0022-1759(84)90040-1.
5
Enzyme immunoassay in which a myeloma protein is used for detection of salmonellae.一种酶免疫测定法，其中使用一种骨髓瘤蛋白来检测沙门氏菌。
Appl Environ Microbiol. 1983 Jun;45(6):1816-21. doi: 10.1128/aem.45.6.1816-1821.1983.
6
Accelerated procedure for Salmonella detection in dried foods and feeds involving only borth cultures and serological reactions.仅涉及肉汤培养和血清学反应的干制食品和饲料中沙门氏菌检测的加速程序。
Appl Microbiol. 1969 Apr;17(4):533-9. doi: 10.1128/am.17.4.533-539.1969.
7
Method for the isolation of highly purified Salmonella flagellins.高纯度沙门氏菌鞭毛蛋白的分离方法。
J Clin Microbiol. 1985 Dec;22(6):1040-4. doi: 10.1128/jcm.22.6.1040-1044.1985.
8
A novel coloured latex test for the detection and identification of more than one antigen.
J Immunol Methods. 1987 Mar 12;97(2):153-8. doi: 10.1016/0022-1759(87)90454-6.
9
Enzyme immunoassay for detection of Salmonella in foods: collaborative study.
J Assoc Off Anal Chem. 1986 Sep-Oct;69(5):786-98.
10
An evaluation of a commercially available enzyme immunoassay test for the rapid detection of salmonellae in food and environmental samples.对一种用于快速检测食品和环境样本中沙门氏菌的商用酶免疫分析测试的评估。
Epidemiol Infect. 1987 Aug;99(1):127-36. doi: 10.1017/s0950268800066930.
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