Lee H A, Wyatt G M, Bramham S, Morgan M R
AFRC Institute of Food Research, Norwich Laboratory, United Kingdom.
Appl Environ Microbiol. 1990 Jun;56(6):1541-6. doi: 10.1128/aem.56.6.1541-1546.1990.
A microtitration plate, antibody-capture, enzyme-linked immunosorbent assay was developed for detection of Salmonella typhimurium. The assay utilizes a monoclonal detector antibody which shows no cross-reactions with non-Salmonella species and only a slight cross-reaction with one other Salmonella serotype. By using only one cultural stage (in a nonselective, chemically defined medium) prior to the enzyme-linked immunosorbent assay, low numbers of cells in food (10 cells 25 g-1) were detected in 19 h. Non-Salmonella competing organisms did not interfere with detection of S. typhimurium even when present in the ratio of 10(6):1 (non-Salmonella/Salmonella spp.). The assay shows the feasibility of rapid, 1-day testing for Salmonella spp. with antibody technology.
开发了一种用于检测鼠伤寒沙门氏菌的微量滴定板抗体捕获酶联免疫吸附测定法。该测定法使用一种单克隆检测抗体，该抗体与非沙门氏菌属无交叉反应，仅与另一种沙门氏菌血清型有轻微交叉反应。通过在酶联免疫吸附测定之前仅使用一个培养阶段（在非选择性化学限定培养基中），在19小时内检测到食品中低数量的细胞（10个细胞/25 g-1）。即使非沙门氏菌竞争菌以10(6):1（非沙门氏菌/沙门氏菌属）的比例存在，也不会干扰鼠伤寒沙门氏菌的检测。该测定法显示了使用抗体技术对沙门氏菌属进行快速一日检测的可行性。
