加拿大流感嗜血杆菌药敏试验方法的监测，包括纸片扩散法的评估。
Surveillance of susceptibility testing methodologies for Haemophilus influenzae in Canada, including evaluation of disk diffusion test.
作者信息
Scriver S R, Hoban D J, McGeer A, Moore T C, Walmsley S L, Low D E
机构信息
Department of Microbiology, Mount Sinai Hospital, University of Toronto, Ontario, Canada.
出版信息
J Clin Microbiol. 1994 Aug;32(8):2013-5. doi: 10.1128/jcm.32.8.2013-2015.1994.
Abstract
We surveyed 75 clinical laboratories to determine if National Committee for Clinical Laboratory Standards (NCCLS) were being used for the susceptibility testing of Haemophilus influenzae. Of the 66 laboratories that performed susceptibility testing, all claimed to follow current NCCLS guidelines. However, upon further questioning, only 23, all of which used disk diffusion testing, accurately interpreted and followed the guidelines. Proficiency testing of 22 of these laboratories found that an unacceptable number of interpretive errors (> 10%) occurred. These results query the merit of routine disk diffusion susceptibility testing of H. influenzae to beta-lactam agents.
摘要
我们调查了75家临床实验室，以确定是否采用了美国国家临床实验室标准委员会（NCCLS）的标准来进行流感嗜血杆菌的药敏试验。在进行药敏试验的66家实验室中，所有实验室均声称遵循现行的NCCLS指南。然而，经进一步询问，只有23家（均采用纸片扩散法检测）准确地解释并遵循了该指南。对其中22家实验室的能力验证发现，存在数量不可接受的解释错误（>10%）。这些结果对流感嗜血杆菌针对β-内酰胺类药物进行常规纸片扩散药敏试验的价值提出了质疑。
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