与单克隆抗体A7的人/鼠嵌合Fab片段偶联的新制癌菌素对人胰腺癌的抗肿瘤作用。
Antitumor effect of neocarzinostatin conjugated to human/mouse chimeric Fab fragments of the monoclonal antibody A7 on human pancreatic carcinoma.
作者信息
Otsuji E, Yamaguchi T, Yata Y, Nishi H, Okamoto K, Taniguchi K, Kato M, Kotani T, Yamaoka N, Kitamura K
机构信息
First Department of Surgery, Kyoto Prefectural University of Medicine, Japan.
出版信息
J Surg Oncol. 1994 Dec;57(4):230-4. doi: 10.1002/jso.2930570405.
The anticancer agent neocarzinostatin (NCS) was bound covalently to human/mouse chimeric Fab fragments of the monoclonal antibody A7 to form the conjugate chA7Fab-NCS. The antitumor effect of chA7Fab-NCS was tested by measuring the inhibition of 3H-thymidine incorporation into human pancreatic carcinoma cells. The chA7Fab-NCS was approximately 2.3 times as effective as free NCS against human pancreatic carcinoma cells which reacted with the monoclonal antibody A7. The antitumor activity of chA7Fab-NCS was inhibited by excess chA7Fab. ChA7Fab-NCS had an antitumor effect equivalent to free NCS on human pancreatic carcinoma cells which did not react with the monoclonal antibody A7. ChA7Fab-NCS appears to be a potentially useful conjugate for immunotargeting chemotherapy against pancreatic carcinoma.
抗癌药物新制癌菌素（NCS）与单克隆抗体A7的人/鼠嵌合Fab片段共价结合，形成偶联物chA7Fab-NCS。通过测量3H-胸腺嘧啶核苷掺入人胰腺癌细胞的抑制率来测试chA7Fab-NCS的抗肿瘤效果。对于与单克隆抗体A7反应的人胰腺癌细胞，chA7Fab-NCS的有效性约为游离NCS的2.3倍。过量的chA7Fab可抑制chA7Fab-NCS的抗肿瘤活性。对于不与单克隆抗体A7反应的人胰腺癌细胞，chA7Fab-NCS具有与游离NCS相当的抗肿瘤效果。chA7Fab-NCS似乎是一种潜在有用的用于胰腺癌免疫靶向化疗的偶联物。
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