眼支原体ISM1499基因组的物理图谱及用于宏观限制性染色体图谱分析的Tn4001衍生物的构建。
Physical map of the genome of Acholeplasma oculi ISM1499 and construction of a Tn4001 derivative for macrorestriction chromosomal mapping.
作者信息
Tigges E, Minion F C
机构信息
Veterinary Medical Research Institute, Iowa State University, Ames 50011.
出版信息
J Bacteriol. 1994 Feb;176(4):1180-3. doi: 10.1128/jb.176.4.1180-1183.1994.
Abstract
A physical chromosomal map of Acholeplasma oculi ISM1499 was constructed by using field inversion gel electrophoresis. To assist in the ordering of the chromosomal fragments, a modified transposon, Tn4001.1064, was constructed. It was also used to rescue mycoplasmal chromosomal sequences adjacent to transposon insertion sites in a one-step cloning procedure. The total size of the A. oculi ISM1499 genome was estimated to be 1,633 kb. The restriction enzyme sites for ApaI, BssHII, EagI, and SmaI were positioned on the map along with several transposon insertion sites.
摘要
利用脉冲场凝胶电泳构建了眼支原体ISM1499的物理染色体图谱。为了辅助染色体片段的排序，构建了一种改良转座子Tn4001.1064。它还用于在一步克隆程序中拯救与转座子插入位点相邻的支原体染色体序列。估计眼支原体ISM1499基因组的总大小为1633kb。ApaI、BssHII、EagI和SmaI的限制性酶切位点以及几个转座子插入位点定位在图谱上。
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