Kopper L, Lapis K, Schaff Z, Mihalik R
First Institute of Pathology and Experimental Cancer Research, Semmelweis Medical University, Budapest, Hungary.
Acta Biomed Ateneo Parmense. 1993;64(5-6):165-9.
The nuclear DNA content of 26 liver tumors (1 adenoma, 10 focal nodular hyperplasia and 15 hepatocellular carcinoma) was measured by flow cytometry (FCM) on formalin-fixed, paraffin-embedded tissues. All lesions developed in noncirrhotic liver and were negative for HBsAg. DNA aneuploidy was found in 1/10 FNH (one hypodiploid) and in 11/15 HCC (73%). Intratumoral heterogeneity was observed in three cases. In HCC the ploidy level showed no correlation with tumor size or histopathological grading. Results support the potential of FCM to measure DNA content in archival pathological specimens, and also that DNA content itself has no diagnostic value.
采用流式细胞术（FCM）对26例肝脏肿瘤（1例腺瘤、10例局灶性结节性增生和15例肝细胞癌）经福尔马林固定、石蜡包埋组织的核DNA含量进行检测。所有病变均发生于非肝硬化肝脏，且HBsAg均为阴性。在10例局灶性结节性增生中有1例（1/10）发现DNA非整倍体（1例亚二倍体），在15例肝细胞癌中有11例（11/15, 73%）发现DNA非整倍体。在3例中观察到瘤内异质性。在肝细胞癌中，倍体水平与肿瘤大小或组织病理学分级无关。结果支持FCM在检测存档病理标本中DNA含量方面的潜力，同时也表明DNA含量本身没有诊断价值。
