Sudhakar Natarajan, Nancy Nirmala Karunakaran, Rajalekshmy Kamalalayam Raghavan, Rajkumar Thangarajan
Department of Molecular Oncology, Cancer Institute, Chennai, Tamil Nadu, India.
Iran J Immunol. 2009 Sep;6(3):141-6.
Precursor B-Acute Lymphoblastic Leukemia (precursor B-ALL) occurs due to the uncontrolled proliferation of B-lymphoid precursors arrested at a particular stage of B-cell development. Precursor-B-ALL is classified mainly into pro-B-ALL, common-ALL and pre-B-ALL. The Common Acute Lymphoblastic Antigen CD10 is the marker for common-ALL.
This study was aimed to examine the diversity of T-cell receptor Gamma (TCRG) and T-cell receptor Delta (TCRD) gene rearrangements in South Indian Common-ALL patients.
Clonality of TCRG and TCRD was studied in 52 cases (pediatric=41 and adolescents and young adults=11) of common-ALL. TCRG and TCRD gene rearrangements were amplified by PCR and the clonality was assessed by Heteroduplex analysis of amplified products.
In pediatric common-ALL, clonal TCRG and TCRD gene rearrangements were detected in 19 (46.3%) and 18 (43.9%) cases respectively. In adolescents and young adults (AYA), TCRG was rearranged in 8 (72.7%) cases and TCRD was rearranged in 4 (36.3%) cases. In the present study of common-ALL, the frequency of a TCRG rearrangement VII-J1.3/2.3 was significantly high in AYA compared to pediatric (36.3% vs 4.8%; p<0.025). Thus, VII-J1.3/2.3 was highly diverse in AYA compared to pediatric. That shows the difference in biology of the disease between pediatric and AYA in South Indian population.
The reason for the high frequency of VII-J1.3/2.3 in AYA of common-ALL in South Indian population in connection with unknown infectious agents or environmental carcinogens needs to be evaluated further.
前体B淋巴细胞急性淋巴细胞白血病（precursor B-ALL）是由于B淋巴细胞前体在B细胞发育的特定阶段停滞并发生不受控制的增殖所致。前体B-ALL主要分为前B-ALL、普通型ALL和前B-ALL。普通急性淋巴细胞抗原CD10是普通型ALL的标志物。
本研究旨在检测南印度普通型ALL患者中T细胞受体γ（TCRG）和T细胞受体δ（TCRD）基因重排的多样性。
对52例普通型ALL患者（儿童41例，青少年及青年成人11例）的TCRG和TCRD克隆性进行研究。通过PCR扩增TCRG和TCRD基因重排，并通过对扩增产物进行异源双链分析评估克隆性。
在儿童普通型ALL中，分别在19例（46.3%）和18例（43.9%）中检测到克隆性TCRG和TCRD基因重排。在青少年及青年成人（AYA）中，8例（72.7%）出现TCRG重排，4例（36.3%）出现TCRD重排。在本普通型ALL研究中，与儿童相比，AYA中TCRG重排VII-J1.3/2.3的频率显著更高（36.3%对4.8%；p<0.025）。因此，与儿童相比，VII-J1.3/2.3在AYA中高度多样。这表明南印度人群中儿童和AYA在该疾病生物学特性上存在差异。
南印度人群普通型ALL的AYA中VII-J1.3/2.3频率高与未知感染因子或环境致癌物相关的原因需要进一步评估。
