Todd J C, Mollitt D L
Department of Surgery, University of Florida Health Science Center, Jacksonville.
Am Surg. 1994 Dec;60(12):954-7.
Sepsis is known to alter red blood cell (RBC) deformability, and this change in flexibility may play a role in the pathophysiology of the hemodynamic alterations characteristic of the septic syndrome. The etiology of this red cell change is unclear. This study evaluates erythrocyte size and cell membrane fluidity during clinical (septic surgical patients) and experimental (endotoxin incubation) sepsis. Membrane lipid viscosity was assessed by fluorescent spectroscopy. Mean corpuscular volume and hemoglobin concentration was determined by automated counter. There was a significant increase in erythrocyte membrane lipid viscosity (P < 0.001) in both the clinical and experimental septic models. No difference was detected, however, in RBC mean corpuscular volume or hemoglobin concentration. These findings suggest that sepsis-induced alterations in erythrocyte deformability are due primarily to an increase in the membrane lipid viscosity and are unrelated to alterations in the cell surface area to volume ratio.
已知脓毒症会改变红细胞（RBC）的可变形性，而这种柔韧性的变化可能在脓毒症综合征特征性血流动力学改变的病理生理学中起作用。这种红细胞变化的病因尚不清楚。本研究评估了临床（脓毒症手术患者）和实验（内毒素孵育）性脓毒症期间的红细胞大小和细胞膜流动性。通过荧光光谱法评估膜脂质粘度。通过自动计数器测定平均红细胞体积和血红蛋白浓度。在临床和实验性脓毒症模型中，红细胞膜脂质粘度均显著增加（P < 0.001）。然而，在红细胞平均体积或血红蛋白浓度方面未检测到差异。这些发现表明，脓毒症诱导的红细胞可变形性改变主要是由于膜脂质粘度增加，与细胞表面积与体积比的改变无关。
