Melkonian A D, Gaylor J D, Cousins R B, Grant M H
Bioengineering Unit, University of Strathclyde, Glasgow, Scotland.
Artif Organs. 1994 Aug;18(8):611-7. doi: 10.1111/j.1525-1594.1994.tb03386.x.
Much effort has been directed toward the development of serum-free, hormonally defined culture conditions for the maintenance of differentiated functions in many cell types, including hepatocytes. However, in the development of a hepatocyte bioreactor for artificial liver support, many designs propose the maintenance of cells in plasma as opposed to defined culture medium. There is very little reported literature on the growth and function of cells cultured in plasma or serum; therefore, the effect of increasing serum concentrations was investigated using the human hepatoma, Hep G2, as a model cell line. It was found that Hep G2 can survive and grow in 100% serum if the serum is supplemented with L-glutamic acid, glycine, and L-cysteine.
为了在包括肝细胞在内的多种细胞类型中维持分化功能，人们付出了很多努力来开发无血清、激素成分明确的培养条件。然而，在用于人工肝支持的肝细胞生物反应器的开发中，许多设计方案建议将细胞维持在血浆中，而非成分明确的培养基中。关于在血浆或血清中培养的细胞的生长和功能，报道的文献非常少；因此，以人肝癌细胞系Hep G2作为模型细胞系，研究了增加血清浓度的影响。结果发现，如果在血清中添加L-谷氨酸、甘氨酸和L-半胱氨酸，Hep G2细胞可以在100%血清中存活和生长。
