Koudelová J, Mourek J
Institute of Physiology, First Faculty of Medicine, Charles University, Prague, Czech Republic.
Physiol Res. 1994;43(3):169-73.
The effect of normobaric oxygen atmosphere on hypoxia-enhanced lipid peroxidation in the brain cortex, subcortical structures, medulla oblongata and in the cerebellum was observed in 7- and 21-day-old and adult rats. The production of free oxygen radicals causing lipid peroxidation was assessed by the method described by Ohkawa et al. (1979). The rats were exposed for 30 min to 100% oxygen atmosphere which significantly stimulated the production of malondialdehyde (MDA) in all the studied regions of the brain in 7- and 21-day-old male rats, and in the brain cortex and subcortical structures of adult males. Higher levels of MDA were found in the brain cortex of 7-day-old female rats only. Reoxygenation with pure oxygen after 30 min hypobaric hypoxia corresponding to 9000 m increased MDA production in all studied parts of the brain on both male and female rats 7- and 21-day-old. In adult rats significantly increased MDA production was only found in the brain cortex of male and female rats and in the subcortical structures of males. The exposition to hypobaric hypoxia followed by reoxygenation by atmospheric air enhanced MDA production in all studied regions of the brain in 7-day-old males and in the cerebellum of females; in 21-day-old rats of both sexes a significant increase of MDA was detected in all parts of the brain. In adult rats were found higher MDA levels in the cerebral cortex of both males and females.
在7日龄、21日龄和成年大鼠中，观察了常压氧环境对大脑皮层、皮层下结构、延髓和小脑中缺氧增强的脂质过氧化作用的影响。采用Ohkawa等人（1979年）描述的方法评估引起脂质过氧化的游离氧自由基的产生。将大鼠暴露于100%氧气环境30分钟，这显著刺激了7日龄和21日龄雄性大鼠以及成年雄性大鼠大脑所有研究区域中丙二醛（MDA）的产生。仅在7日龄雌性大鼠的大脑皮层中发现了较高水平的MDA。在相当于9000米的30分钟低压缺氧后用纯氧复氧，增加了7日龄和21日龄雄性和雌性大鼠大脑所有研究部位的MDA产生。在成年大鼠中，仅在雄性和雌性大鼠的大脑皮层以及雄性的皮层下结构中发现MDA产生显著增加。在7日龄雄性大鼠的大脑所有研究区域以及雌性大鼠的小脑中，暴露于低压缺氧后再用大气空气复氧会增加MDA的产生；在21日龄的两性大鼠中，大脑所有部位均检测到MDA显著增加。在成年大鼠中，雄性和雌性大鼠的大脑皮层中均发现较高的MDA水平。
